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      AGO1 enhances the proliferation and invasion of cholangiocarcinoma via the EMT-associated TGF-β signaling pathway

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          Abstract

          Purpose: To detect the expression of AGO1 in cholangiocarcinoma and explore its role and significance in the progression of cholangiocarcinoma. Patients and methods: Using immunohistochemistry, Western blotting and qPCR, we examined the expression of AGO1 in cholangiocarcinoma tissues. Through the analysis of clinical case data, the relationship between AGO1 and clinical prognosis was explored. The effect of AGO1 on cholangiocarcinoma was verified by cell functional experiments. Finally, we examined the effects of AGO1 on EMT-related proteins and signaling pathways. Results: AGO1 is significantly upregulated in cholangiocarcinoma and is associated with the prognosis of cholangiocarcinoma. AGO1 can significantly increase the proliferation, migration and invasion of cholangiocarcinoma cell lines. AGO1 affects the prognosis of cholangiocarcinoma by affecting epithelial-mesenchymal transition (EMT)-related TGF-β-PI3K-AKT signaling pathways. Conclusion: AGO1 is an independent predictor of cholangiocarcinoma prognosis and a potential target for the treatment of cholangiocarcinoma.

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          Author and article information

          Journal
          Am J Transl Res
          Am J Transl Res
          ajtr
          American Journal of Translational Research
          e-Century Publishing Corporation
          1943-8141
          2020
          15 June 2020
          : 12
          : 6
          : 2890-2902
          Affiliations
          [1 ] Department of General Surgery, The First Affiliated Hospital of University of Science and Technology of China Hefei, Anhui, China
          [2 ] Anhui Provincial Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of University of Science and Technology of China Hefei, Anhui, China
          [3 ] Perelman School of Medicine, University of Pennsylvania Pennsylvania, PA, USA
          Author notes
          Address correspondence to: Chao Wang, Department of General Surgery, The First Affiliated Hospital of University of Science and Technology of China, No. 17, Lu Jiang Road, Hefei, Anhui, China. Tel: +86-18553696106; E-mail: ahslyywc@ 123456sohu.com
          [*]

          Equal contributors.

          Article
          PMC7344082 PMC7344082 7344082
          7344082
          32655817
          cd05131c-c909-4a48-a8c9-1ab33b23be5a
          AJTR Copyright © 2020
          History
          : 10 September 2019
          : 22 January 2020
          Categories
          Original Article

          prognosis,AGO1,epithelial-mesenchymal transition (EMT),Cholangiocarcinoma (CCA)

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