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      Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group

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          Abstract

          Premise

          Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae).

          Methods and Results

          Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross‐species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers.

          Conclusions

          Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group.

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          A rapid DNA isolation procedure for small quantities of fresh leaf tissue

          JJ, Doyle, JJ Doyle, JD Doyle … (1987)
          Article 0 comments Cited 1085 times – based on 0 reviews     Review now
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            Conservation genetics of the endangered endemic Hawaiian genus Brighamia (Campanulaceae).

            D. Ragone, S. Perlman, K. Wood … (1998)
            The endemic Hawaiian genus Brighamia (Campanulaceae) comprises two federally endangered, morphologically similar species, B. insignis from Kaua`i and Ni`ihau and B. rockii from Moloka`i. To assist the design of conservation management programs for these taxa, isozyme analyses were performed to assess the levels of genetic diversity at the population and species levels, including comparisons within and among seven natural populations and one ex situ collection each of B. insignis and B. rockii. Our sampling (N = 80) represents ~41% of all known individuals in the wild. Isozyme analyses revealed levels of genetic variation comparable to those reported for other Hawaiian flowering plant taxa but low levels of genetic variation at the population and species levels when compared to flowering plants in general. Ex situ individuals (N = 61) were genetically representative of natural populations and hence may appropriately serve as stock for population augmentations. The two morphologically similar Brighamia species were highly distinct genetically. The combination of morphological and ecological similarity with allozymic dissimilarity observed in Brighamia is unique among the Hawaiian taxa studied to date.
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              GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research

              R Peakall, PE SMOUSE, P. SMOUSE … (2006)
              Article 0 comments Cited 6 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Jeremie B. Fant:
                ORCID: https://orcid.org/0000-0001-9276-1111
                jfant@chicagobotanic.org
                Journal
                Journal ID (nlm-ta): Appl Plant Sci
                Journal ID (iso-abbrev): Appl Plant Sci
                Journal ID (doi): 10.1002/(ISSN)2168-0450
                Journal ID (publisher-id): APS3
                Title: Applications in Plant Sciences
                Publisher: John Wiley and Sons Inc. (Hoboken )
                ISSN (Electronic): 2168-0450
                Publication date (Electronic): 11 November 2019
                Publication date Collection: November 2019
                Volume: 7
                Issue: 11 ( doiID: 10.1002/aps3.v7.11 )
                Electronic Location Identifier: e11303
                Affiliations
                [ 1 ] Plant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USA
                [ 2 ] Plant Biology and Conservation Northwestern University O. T. Hogan Hall, 2205 Tech Drive Evanston Illinois 60208 USA
                [ 3 ] University of Illinois at Urbana‐Champaign Champaign Illinois 61820 USA
                Author notes
                [*] [* ]Author for correspondence: jfant@ 123456chicagobotanic.org
                Author information
                https://orcid.org/0000-0001-9276-1111
                Article
                Publisher ID: APS311303
                DOI: 10.1002/aps3.11303
                PMC ID: 6858292
                SO-VID: cd24a97a-c822-4af6-883f-2f5e25e75547
                Copyright © © 2019 Fant et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America
                License:

                This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

                History
                Date received : 20 August 2019
                Date accepted : 22 September 2019
                Page count
                Figures: 0, Tables: 3, Pages: 6, Words: 5888
                Funding
                Funded by: Eppley Foundation
                Funded by: Institute of Museum and Library Services , open-funder-registry 10.13039/100000208;
                Award ID: MG‐30‐16‐0085‐16
                Funded by: Chicago Botanic Garden College First Program
                Funded by: Charles and Margery Barancik Foundation
                Funded by: U.S. National Science Foundation Research Experiences for Undergraduates (NSF-REU)
                Award ID: DEB‐1757800
                Categories
                Subject: Primer Note
                Subject: Primer Notes
                Custom metadata
                source-schema-version-number 2.0
                cover-date November 2019
                details-of-publishers-convertor Converter:WILEY_ML3GV2_TO_JATSPMC version:5.7.2 mode:remove_FC converted:15.11.2019

                Keywords: brighamia,campanulaceae,cross‐amplification,hawai‘ian lobelioids,lobelia,microsatellites
                Data availability:
                Keywords: brighamia, campanulaceae, cross‐amplification, hawai‘ian lobelioids, lobelia, microsatellites

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