Protective Effects of Extracts and Flavonoids Isolated from  Scutia buxifolia  Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide

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Abstract

Flavonoids are claimed to protect against cardiovascular disease, certain forms of cancer and ageing, possibly by preventing initial DNA damage. Therefore, we investigated the protective effects of crude extract, ethyl acetate fraction and flavonoids (quercetin, quercitrin, isoquercitrin and rutin) isolated from the leaves from Scutia buxifolia against chromosome damage induced by H ₂O ₂in human lymphocytes by analyzing cellular growth rate, cell viability, mitotic index and chromosomal instability. We found a differential response among the compounds tested, with the ethyl acetate fraction being more effective than the crude extract, a difference perhaps related to the presence of the antioxidants identified and quantified by HPLC/DAD. In general, quercetin, isoquercitrin and rutin recovered the mitotic index and chromosomal instability more than quercitrin after treatment with hydrogen peroxide.

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Author and article information

Journal

Journal ID (nlm-ta): Molecules

Journal ID (iso-abbrev): Molecules

Journal ID (publisher-id): molecules

Title: Molecules

Publisher: MDPI

ISSN (Electronic): 1420-3049

Publication date (Electronic): 14 May 2012

Publication date Collection: May 2012

Volume: 17

Issue: 5

Pages: 5757-5769

Affiliations

[1 ]Program of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil

[2 ]Franciscan University Center – UNIFRA, Andradas Street, 1614, Santa Maria, RS 97010-032, Brazil

[3 ]Program of Post-Graduation in Pharmacology, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil

[4 ]Federal University of Pampa – UNIPAMPA, Uruguaiana, RS 97500-970, Brazil

Author notes

[* ] Author to whom correspondence should be addressed; Email: alineboligon@ 123456hotmail.com ; Tel.: +55-55-3220-9618.

Article

Publisher ID: molecules-17-05757

DOI: 10.3390/molecules17055757

PMC ID: 6268969

PubMed ID: 22628047

SO-VID: cd41df00-3c52-4cfc-95f2-84dffd46f390

License:

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

History

Date received : 26 March 2012

Date revision received : 25 April 2012

Date accepted : 26 April 2012

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Protective Effects of Extracts and Flavonoids Isolated from Scutia buxifolia Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide

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