We report Talitroides alluaudi (Chevreux, 1896) from Miyako Island, Ryukyu Islands, Japan. Although this terrestrial amphipod is distributed worldwide, including Indo-Pacific islands, Europe, and North and South America, the present specimens represent the first record of this species from Japanese islands. The cytochrome c oxidase subunit I (COI) sequence of a Miyako Island specimen was unique compared with the known COI sequences from Taiwanese and Bermudan populations.