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      Expression of integrins to control migration direction of electrotaxis

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          Abstract

          Proper control of cell migration is critically important in many biologic processes, such as wound healing, immune surveillance, and development. Much progress has been made in the initiation of cell migration; however, little is known about termination and sometimes directional reversal. During active cell migration, as in wound healing, development, and immune surveillance, the integrin expression profile undergoes drastic changes. Here, we uncovered the extensive regulatory and even opposing roles of integrins in directional cell migration in electric fields (EFs), a potentially important endogenous guidance mechanism. We established cell lines that stably express specific integrins and determined their responses to applied EFs with a high throughput screen. Expression of specific integrins drove cells to migrate to the cathode or to the anode or to lose migration direction. Cells expressing αMβ2, β1, α2, αIIbβ3, and α5 migrated to the cathode, whereas cells expressing β3, α6, and α9 migrated to the anode. Cells expressing α4, αV, and α6β4 lost directional electrotaxis. Manipulation of α9 molecules, one of the molecular directional switches, suggested that the intracellular domain is critical for the directional reversal. These data revealed an unreported role for integrins in controlling stop, go, and reversal activity of directional migration of mammalian cells in EFs, which might ensure that cells reach their final destination with well-controlled speed and direction.—Zhu, K., Takada, Y., Nakajima, K., Sun, Y., Jiang, J., Zhang, Y., Zeng, Q., Takada, Y., Zhao, M. Expression of integrins to control migration direction of electrotaxis.

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          Author and article information

          Journal
          FASEB J
          FASEB J
          fasebj
          fasebj
          The FASEB Journal
          Federation of American Societies for Experimental Biology (Bethesda, MD, USA )
          0892-6638
          1530-6860
          August 2019
          22 May 2019
          22 May 2020
          : 33
          : 8
          : 9131-9141
          Affiliations
          [* ]Department of Dermatology, School of Medicine, University of California–Davis, Sacramento, California, USA;
          []Institute for Regenerative Cures, University of California–Davis, Sacramento, California, USA;
          []State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Surgery Research, Third Military Medical University, Chongqing, China;
          [§ ]Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China;
          []Department of Ophthalmology and Vision Science, School of Medicine, University of California–Davis, Sacramento, California, USA
          Author notes
          [1 ]Correspondence: Department of Dermatology, School of Medicine, University of California–Davis, 4645 Second Ave., Research III Suite 3300, Sacramento, CA 95817, USA. E-mail: ytakada@ 123456ucdavis.edu
          [2 ]Correspondence: Institute for Regenerative Cures, University of California–Davis, 2921 Stockton Blvd., Sacramento, CA 95817, USA. E-mail: minzhao@ 123456ucdavis.edu
          Article
          PMC6662972 PMC6662972 6662972 FJ_201802657R
          10.1096/fj.201802657R
          6662972
          31116572
          cd84dc98-fa95-4459-96e3-b9243749a50b
          © FASEB
          History
          : 08 December 2018
          : 15 April 2019
          Page count
          Figures: 8, Tables: 0, Equations: 0, References: 73, Pages: 11
          Categories
          Research
          Custom metadata
          v1

          directional cell migration,motility,α9,galvanotaxis,directional reversal

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