Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22( N), 23, 25(26)-trien-3 β-ol-3- O- β-D-glucopyranosyl-(1→2)- β-D-glucopyranosyl-(1→4)- β-D-galactopyranoside ( 1) and 26- O- β-D-glucopyranosyl-(25 R)-5 α-furost-20(22)-en-3 β, 26-diol ( 2), together with 7 known ones including 26- O- β-D-glucopyranosyl-(25 R)-5, 20(22)-dien-furost-3 β, 26-diol ( 3), (25 R)-5-en-spirost-3 β-ol- O- β-D-glucopyranosyl-(1→4)-[ α-L-rhmanopyranosyl-(1→2)]- β-D-galactopyranoside ( 4), funkioside D ( 5), aspidistrin ( 6), tigogenin-3- O- β-D-lucotrioside ( 7), desglucolanatigonin II ( 8), and degalactotigonin ( 9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides ( 6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.