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      A prokineticin-like protein responds to immune challenges in the gastropod pest Pomacea canaliculata.

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          Abstract

          The golden apple snail Pomacea canaliculata is an invasive pest originating from South America. It has already been found in Asia, the southern United States and more recently in the EU. Aiming to target the immune system of the snail as a way to control its spreading, we have developed organ-specific transcriptomes and looked for molecules controlling replication and differentiation of snail hemocytes. The prokineticin domain-containing protein Astakine 1 is the only cytokine known thus far capable of regulating invertebrate hematopoiesis, and we analyzed the transcriptomes looking for molecules containing a prokineticin domain. We have identified a prokineticin-like protein (PlP), that we called Pc-plp and we analyzed by real-time PCR (qPCR) its expression. In control snails, highest levels of Pc-plp were detected in the digestive gland, the ampulla (i.e., a hemocyte reservoir) and the pericardial fluid (i.e., the hematopoietic district). We tested Pc-plp expression after triggering hematopoiesis via multiple hemolymph withdrawals, or during bacterial challenge through LPS injection. In both cases a reduction of Pc-plp mRNA was observed. The multiple hemolymph withdrawals caused a significant decrease of Pc-plp mRNA in pericardial fluid and circulating hemocytes, while the LPS injection promoted the Pc-plp mRNA drop in anterior kidney, mantle and gills, organs that may act as immune barrier in molluscs. Our data indicate an important role for prokineticin domain-containing proteins as immunomodulators also in gastropods and their dynamic expression may serve as a biosensor to gauge the effectiveness of immunological interventions aimed at curtailing the spreading of the gastropod pest P. canaliculata.

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          Author and article information

          Journal
          Dev. Comp. Immunol.
          Developmental and comparative immunology
          Elsevier BV
          1879-0089
          0145-305X
          Jul 2017
          : 72
          Affiliations
          [1 ] Stowers Institute for Medical Research, Kansas City, MO, USA; Howard Hughes Medical Institute, Stowers Institute for Medical Research, Kansas City, MO, USA.
          [2 ] Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia, Modena, Italy.
          [3 ] Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy.
          [4 ] Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy. Electronic address: davide.malagoli@unimore.it.
          Article
          S0145-305X(16)30462-1
          10.1016/j.dci.2017.02.001
          28163091
          cdb64ee4-d1f3-4ae6-ad95-d93b3e19678b
          History

          Astakine,Cytokine,Hematopoiesis regulation,Hemolymph,LPS,Molluscan hemocytes,Pomacea immunity

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