12
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Characterization of the first complete genome sequence of an Impatiens necrotic spot orthotospovirus isolate from the United States and worldwide phylogenetic analyses of INSV isolates

      brief-report

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Objective

          Impatiens necrotic spot orthotospovirus (INSV) can impact economically important ornamental plants and vegetables worldwide. Characterization studies on INSV are limited. For most INSV isolates, there are no complete genome sequences available. This lack of genomic information has a negative impact on the understanding of the INSV genetic diversity and evolution. Here we report the first complete nucleotide sequence of a US INSV isolate.

          Results

          INSV-UP01 was isolated from an impatiens in Pennsylvania, US. RT-PCR was used to clone its full-length genome and Vector NTI to assemble overlapping sequences. Phylogenetic trees were constructed by using MEGA7 software to show the phylogenetic relationships with other available INSV sequences worldwide. This US isolate has genome and biological features classical of INSV species and clusters in the Western Hemisphere clade, but its origin appears to be recent. Furthermore, INSV-UP01 might have been involved in a recombination event with an Italian isolate belonging to the Asian clade. Our analyses support that INSV isolates infect a broad plant-host range they group by geographic origin and not by host, and are subjected to frequent recombination events. These results justify the need to generate and analyze complete genome sequences of orthotospoviruses in general and INSV in particular.

          Electronic supplementary material

          The online version of this article (10.1186/s13104-018-3395-5) contains supplementary material, which is available to authorized users.

          Related collections

          Most cited references44

          • Record: found
          • Abstract: found
          • Article: not found

          Insect vector interactions with persistently transmitted viruses.

          The majority of described plant viruses are transmitted by insects of the Hemipteroid assemblage that includes aphids, whiteflies, leafhoppers, planthoppers, and thrips. In this review we highlight progress made in research on vector interactions of the more than 200 plant viruses that are transmitted by hemipteroid insects beginning a few hours or days after acquisition and for up to the life of the insect, i.e., in a persistent-circulative or persistent-propagative mode. These plant viruses move through the insect vector, from the gut lumen into the hemolymph or other tissues and finally into the salivary glands, from which these viruses are introduced back into the plant host during insect feeding. The movement and/or replication of the viruses in the insect vectors require specific interactions between virus and vector components. Recent investigations have resulted in a better understanding of the replication sites and tissue tropism of several plant viruses that propagate in insect vectors. Furthermore, virus and insect proteins involved in overcoming transmission barriers in the vector have been identified for some virus-vector combinations.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences

            Background The rapid increase in the amount of protein and DNA sequence information available has become almost overwhelming to researchers. So much information is now accessible that high-quality, functional gene analysis and categorization has become a major goal for many laboratories. To aid in this categorization, there is a need for non-commercial software that is able to both align sequences and also calculate pairwise levels of similarity/identity. Results We have developed MatGAT (Matrix Global Alignment Tool), a simple, easy to use computer application that generates similarity/identity matrices for DNA or protein sequences without needing pre-alignment of the data. Conclusions The advantages of this program over other software are that it is open-source freeware, can analyze a large number of sequences simultaneously, can visualize both sequence alignment and similarity/identity values concurrently, employs global alignment in calculations, and has been formatted to run under both the Unix and the Microsoft Windows Operating Systems. We are presently completing the Macintosh-based version of the program.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Thrips transmission of tospoviruses.

              One hundred years ago, the disease tomato spotted wilt was first described in Australia. Since that time, knowledge of this disease caused by Tomato spotted wilt virus (TSWV) and transmitted by thrips (insects in the order Thysanoptera) has revealed a complex relationship between the virus, vector, plant host, and environment. Numerous tospoviruses and thrips vectors have been described, revealing diversity in plant host range and geographical distributions. Advances in characterization of the tripartite interaction between the virus, vector, and plant host have provided insight into molecular and ecological relationships. Comparison to animal-infecting viruses in the family Bunyaviridae has enabled the identification of commonalities between tospoviruses and other bunyaviruses in transmission by arthropod vectors and molecular interactions with hosts. This review provides a special emphasis on TSWV and Frankliniella occidentalis, the model tospovirus-thrips pathosystem. However, other virus-vector combinations are also of importance and where possible, comparisons are made between different viruses and thrips vectors.
                Bookmark

                Author and article information

                Contributors
                kxz145@psu.edu
                pam17@dsmz.de
                czr2@psu.edu
                Journal
                BMC Res Notes
                BMC Res Notes
                BMC Research Notes
                BioMed Central (London )
                1756-0500
                10 May 2018
                10 May 2018
                2018
                : 11
                : 288
                Affiliations
                [1 ]ISNI 0000 0001 2097 4281, GRID grid.29857.31, Department of Plant Pathology and Environmental Microbiology, , Pennsylvania State University, ; University Park, PA 16802 USA
                [2 ]ISNI 0000 0000 9247 8466, GRID grid.420081.f, Plant Virus Department, , Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, ; 38124 Brunswick, Germany
                Article
                3395
                10.1186/s13104-018-3395-5
                5946465
                29747679
                ce93fa3a-17d7-49f3-a668-b89269b814ec
                © The Author(s) 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 26 February 2018
                : 3 May 2018
                Categories
                Research Note
                Custom metadata
                © The Author(s) 2018

                Medicine
                impatiens necrotic spot orthotospovirus,orthotospovirus,complete genome sequence,phylogenetic analysis,recombination

                Comments

                Comment on this article