Here we present a complete data set on the richness of medium- and large- sized mammals from a fragmented area of the southern Brazilian savanna (Cerrado). We described the frequency, occurrence status and activity period of the most common species. In each remnant, the community of medium- and large-sized mammals was inventoried across the following methods: tracks/prints, vocalizations, sightings, burrows, and camera trap images between August 2016 and January 2017. We recorded 23 species of medium- and large-sized mammals, of which seven are threatened. The study area presented a great potential, where we recorded 45.1% of all medium- and large-sized mammal species occurring in the Cerrado. Myrmecophaga tridactyla, Dasypus novemcinctus, Tapirus terrestris, Cerdocyon thous and Cuniculus paca were classified as common, which was expected since they have large habitat range, and therefore, can be recorded more frequently. Species with large body size, such as T. terrestris, M. tridactyla and C. thous, were also the ones with the greatest variation in time period of activity, occurring both in nocturnal and diurnal time periods. Therefore, our study underscores that this transition area, although fragmented, still maintains an expressive fauna of medium- and large-sized mammals, including endangered species.