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      Effects of husk and harvest time on carotenoid content and acceptability of roasted fresh cobs of orange maize hybrids

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          Abstract

          Vitamin A deficiency (VAD) is a major public health problem in many developing countries. Orange maize is preferred as green maize and consumed roasted on the cob, especially in Nigeria. This research work was to evaluate the effects of harvest time and husk on the carotenoid contents and sensory properties of roasted orange maize hybrids. The results showed that husk (roasting forms) and harvesting time had significant effects ( P ≤ 0.001) on the carotenoids and the sensory properties. There was general increase in β-carotene and provitamin A (PVA) values as the harvesting time increases. The β-carotene and PVA values for roasted orange maize hybrids with husk were higher than those for roasted without husk. Hybrid 5 had the highest β-carotene concentration and PVA value at 27 days after pollination (DAP) and 34DAP when unprocessed and roasted without husk. This information can help researchers in choosing proper roasting methods to increase the retention of high levels of β-carotene and PVA in orange maize that can be delivered to consumers through nutrition education.

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          Most cited references43

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          Quantification of carotenoid and tocopherol antioxidants in Zea mays.

          Recent investigations into carotenoid and tocopherol biological activity in mammalian systems indicate that these antioxidants are associated with the prevention of degenerative diseases. Both carotenoids and tocopherols can be found in corn kernel tissue. A replicated survey of 44 sweet and dent corn lines was conducted to determine qualitative and quantitative variability of lutein, zeaxanthin, beta-cryptoxanthin, alpha-carotene, and beta-carotene, as well as the alpha-, delta-, and gamma- forms of tocopherol. The primary carotenoids in fresh market sweet corn were found to be lutein and zeaxanthin, with the gamma form dominating among the tocopherols. Mean values among the genotypes were observed to range from 0 to 20.0 and 2.4 to 63.3 microg/g dry weight for lutein and gamma-tocopherol, respectively, indicating variability among genotypes in genes regulating the metabolism of these compounds. The observed genetic variability suggests profound differences in potential health promotion among genotypes and supports the feasibility of developing germplasm with enhanced levels of these antioxidant compounds at dosages that could promote health among the consuming public.
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            Dietary diversification/modification strategies to enhance micronutrient content and bioavailability of diets in developing countries.

            Both cereal staples and household diets can be manipulated to enhance the content of micronutrients and/or alter the levels of absorption modifiers to improve micronutrient bioavailability. Strategies described range from plant breeding, use of fertilizers and genetic engineering to changes in food preparation and processing methods at the household level involving soaking, fermentation and germination. The impact of five household strategies designed to enhance the content and bioavailability of iron, zinc and calcium in a representative daily menu for rural Malawian preschool children has been calculated using food composition data. In the five strategies, relishes based on small dried fish replaced plant-based relishes, maize-based porridges prepared with maize flour soaked to reduce its hexa (IP-6)- and penta (IP-5)-inositol phosphate content replaced conventional porridges; and a pumpkin-leaf relish replaced sweet potato to increase the retinol content of the daily menu. Comparison of the calculated energy, nutrient, and phytate content, and [phytate]:[zinc] molar ratios of the five modified menus compared with the unmodified menu emphasizes that to ensure that the estimated requirements for iron and zinc are met, the optimal strategy includes dried fish relish twice daily together with porridges prepared using soaked (or fermented) maize flour to reduce their hexa- and penta-inositol phosphate content. Implementation of these household strategies has the potential to increase the bioavailability of iron and zinc in rural Malawian diets from low to high.
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              Evaluation of analytical methods for carotenoid extraction from biofortified maize (Zea mays sp.).

              Biofortification of maize with beta-carotene has the potential to improve vitamin A status in vitamin A deficient populations where maize is a staple crop. Accurate assessment of provitamin A carotenoids in maize must be performed to direct breeding efforts. The objective was to evaluate carotenoid extraction methods and determine essential steps for use in countries growing biofortified maize. The most reproducible method based on coefficient of variation and extraction efficiency was a modification of Kurilich and Juvik (1999). Heat and saponification are required to release carotenoids from biofortified maize and remove oils interfering with chromatographic analysis. For maize samples with high oil content, additional base may be added to ensure complete saponification without compromising results. Degradation of internal standard before carotenoids were released from the maize matrix required the addition of internal standard after heating to prevent overestimation of carotenoids. This modified method works well for lutein, zeaxanthin, beta-cryptoxanthin, alpha-carotene, and beta-carotene.
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                Author and article information

                Journal
                Food Sci Nutr
                Food Sci Nutr
                fsn3
                Food Science & Nutrition
                BlackWell Publishing Ltd (Oxford, UK )
                2048-7177
                2048-7177
                November 2014
                14 October 2014
                : 2
                : 6
                : 811-820
                Affiliations
                [1 ]International Institute of Tropical Agriculture (IITA) P.M.B. 5320, Oyo Road, Ibadan, Oyo State, Nigeria
                [2 ]Ekiti State University Ado-Ekiti, P.M.B. 5363, Ekiti State, Nigeria
                Author notes
                Correspondence Bussie Maziya-Dixon, International Institute of Tropical Agriculture (IITA), Carolyn House 26 Dingwall Road, Croydon CR9 3EE, United Kingdom. Tel: +234-803-403-5281;, Fax: 44-208-711-3786; E-mail: b.dixon@ 123456cgiar.org

                Funding Information No funding information provided.

                Article
                10.1002/fsn3.179
                4256587
                25493200
                cf84efdf-2a8a-42f4-b785-8503e8514877
                © 2014 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.

                This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                : 06 August 2014
                : 04 September 2014
                : 12 September 2014
                Categories
                Original Research

                harvesting time,husk,orange maize,provitamin a,roasting,β-carotene

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