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      Grass Carp (Ctenopharyngodon idellus) Bile may Inhibit the Release of Renal Dipeptidase from the Proximal Tubules by Nitric Oxide Generation

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          Abstract

          There are many reports on acute renal failure (ARF) after ingestion of grass carp bile (CB; Ctenopharyngodon idellus). Renal dipeptidase (RDPase; EC 3.4.13.19) is a glycosylphosphatidylinositol–anchored ectoenzyme within the renal proximal tubules (PTs) and is proposed as a diagnostic enzyme of renal disease. We examined the release of RDPase following treatment with CB and various nitric oxide (NO) related compounds in porcine PTs. The RDPase release from PTs was inhibited by CB in a concentration–dependent manner and was also inhibited by sodium nitroprusside (direct NO donor) and L–arginine (NO synthase substrate) in the tested range (0–12 m M). CB–treated (0.1 mg/ml) PTs showed a decreased RDPase activity in comparison with the control group. This inhibition was blocked by 2 m M L–NAME (NO synthase inhibitor) and U73122 (inhibitor of phosphatidylinositol–specific phospholipase C) in a concentration–dependent manner. Eel bile (0–0.1 mg/ml), used as the control, did not significantly affect the RDPase release from PTs. The NO concentration was observed as nitrite, the degradation product of the NO metabolism, increased in proportion to CB and L–arginine. The increase of nitrite to 151.5% by CB treatment (0.1 mg/ml) was blocked by 2 m M L–NAME (95.5%). When the phospholipase C pathway was blocked by 10 and 20 μ M U73122, the nitrite generation decreased to 122.7 and 89.4%, respectively. These results strongly suggest that NO generation and the phospholipase C pathway affect the RDPase release from the PTs and that they may be involved in the development of ARF in vivo following CB ingestion. The release of RDPase from PTs could be a useful tool not only for this CB–caused ARF, but also for the elucidation of other biochemical mechanisms.

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          Most cited references 4

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          • Abstract: not found
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          Inhibition of Ecto-5`-nucleotidase by Nitric Oxide Donors

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            Plasma electrolyte changes after ingestion of bile extract of the grass carp (Ctenopharyngodon idellus) in rats

             C.F Chen,  M.C Lin,  H.M. Liu (1990)
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              Toxic material from the gallbladder of the grass carp (Ctenopharyngodon idellus)

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                Author and article information

                Journal
                KBR
                Kidney Blood Press Res
                10.1159/issn.1420-4096
                Kidney and Blood Pressure Research
                S. Karger AG
                1420-4096
                1423-0143
                2000
                2000
                24 March 2000
                : 23
                : 2
                : 113-118
                Affiliations
                aHormone Research Center, bCollege of Pharmacy, cCollege of Natural Science, and dCollege of Veterinary Medicine, Chonnam National University, Kwangju, and eCollege of Medicine, Chonbuk National University, Chonju, Korea
                Article
                25962 Kidney Blood Press Res 2000;23:113–118
                10.1159/000025962
                10765113
                © 2000 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 5, References: 24, Pages: 6
                Product
                Self URI (application/pdf): https://www.karger.com/Article/Pdf/25962
                Categories
                Original Paper

                Cardiovascular Medicine, Nephrology

                Renal dipeptidase, Nitric oxide, Carp bile, Proximal tubules

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