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      Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

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          Abstract

          In this study, the methylation-sensitive amplification polymorphism (MSAP) was used to compare the genomic DNA methylation level of muscle, gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of osmoregulation. Thirty primers were used to analyze the difference of methylation level of different tissues. The results showed that the baseline methylation level of muscle, hepatopancreas and gill was 47.31%, 22.94% and 17.69%, respectively. After exposed to low salinity stress, the methylation epiloci changed in the three tissues. Both demethylation and methylation processes occurred under low salinity stress. The methylation ratio decreased in muscle and gill but increased in hepatopancreas. These results indicated that DNA methylation is tissue-specific when P. trituberculatus responds to low salinity.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          24 August 2019
          01 October 2019
          : 18
          : 5
          : 1158-1162
          Affiliations
          1Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China
          2Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China
          3Third Institute of Oceanography, State Oceanic Administration, Xiamen 361001, China
          Author notes
          *Corresponding author: LI Ronghua
          Article
          s11802-019-4045-9
          10.1007/s11802-019-4045-9
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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