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      CD201 and CD27 identify hematopoietic stem and progenitor cells across multiple murine strains independently of Kit and Sca-1.

      1 , 2 , 3
      Experimental hematology
      Elsevier BV

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          Abstract

          Identification and isolation of hematopoietic stem cells (HSCs) in mice is most commonly based on the expression of surface molecules Kit and Sca-1 and the absence of markers of mature lineages. However, Sca-1 is absent or weakly expressed in hematopoietic progenitors in many strains, including nonobese diabetic (NOD), BALB/c, C3H, and CBA mice. In addition, both Kit and Sca-1 levels are modulated following bone marrow injury. In these cases, other markers and dye exclusion methods have been employed to identify HSCs, yet there is no antibody-based stain that enables identification of HSCs and early progenitors when Kit and Sca-1 are inadequate. CD201 is a marker that is highly restricted to HSCs and progenitors, and CD27 is expressed at moderate-to-high levels on HSCs. We show here that combining CD201 and CD27 enables highly efficient isolation of long-term HSCs in NOD mice as well as in other strains, including SJL, FVB, AKR, BALB/c, C3H, and CBA. We also find that HSCs appear to maintain expression of CD201 and CD27 after hematopoietic injury when Kit expression is downregulated. These results suggest a widely applicable yet simple alternative for HSC isolation in settings where Kit and Sca-1 expression are insufficient.

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          Author and article information

          Journal
          Exp. Hematol.
          Experimental hematology
          Elsevier BV
          1873-2399
          0301-472X
          Jul 2015
          : 43
          : 7
          Affiliations
          [1 ] Department of Immunobiology, Joslin Diabetes Center, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
          [2 ] Sue and Bill Gross Stem Cell Research Center, Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, USA.
          [3 ] Department of Immunobiology, Joslin Diabetes Center, Boston, MA, USA; Harvard Medical School, Boston, MA, USA. Electronic address: thomas.serwold@joslin.harvard.edu.
          Article
          S0301-472X(15)00105-8 NIHMS682032
          10.1016/j.exphem.2015.04.001
          4480781
          25892186
          d011bb1f-96e3-4655-b2c1-7acaae1bafa6
          History

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