The effect of selenium, as selenite, on vanadate-induced ROS generation in CHO-K1 cells measured using dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay

Abstract

Abstract. Objective: Vanadium is an established worldwide environmental pollutant whose toxic effects on living systems represent a growing safety concern. The generation of reactive oxidative species (ROS) is indicated as the main cause of vanadium adverse health effects. However, the knowledge about the potential interactions between vanadium and essential micronutrients with antioxidant activity such as selenium on oxidative stress markers is very restricted. Materials and methods: Herein, we used CHO-K1 cells to find out if vanadium can interact with selenium on the generation of ROS measured with a fluorescein probe, dichloro-dihydro-fluorescein diacetate (DCFH-DA) dye. For the detection of interactive effects, we have analyzed the data with a statistical tool two-way ANOVA. Results: As expected, a marked rise in ROS generation in the cells following 2-. or 24-hour treatment with 100 µM NaVO 3 alone was observed. The two-way ANOVA did not detect significant interaction between 100 µM vanadate and selenite (0.5 µM and 1 µM) on ROS production following 2 and 24 hours co-exposure although a mild tendency (p = 0.117) towards synergistic interactive effects between 100 µM vanadate, and 1 µM selenite emerged after 24 hours of co-exposure. Conclusion: These findings show that selenium, as selenite, may not be beneficial in the treatment of vanadium toxicity.