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      Effects of Escherichia coli Lipopolysaccharide on Renal Glomerular and Tubular Adenylate Cyclase

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          Abstract

          The effects of Escherichia coli lipopolysaccharide (LPS) on adenylate cyclase have been tested using renal tubular membranes and renal glomeruli isolated from rats. E. Coli LPS did not stimulate glomerular and tubular basal adenylate cyclase activity whereas it was an activator in the presence of fluoride. The effect of E. Coli LPS was immediate but was greater after 20 min preincubation. Maximum stimulation of both glomerular and tubular fluoride sensitive adenylate cyclase occurred at 125 µg/ml of E. Coli LPS with an apparent Km (dose corresponding to 50% of maximum stimulation) of 30 µg/ml. Above 125 µg/ml there was a decrease in adenylate cyclase activity. E. Coli LPS produced an increase in the maximum velocity of both enzymes but did not affect their affinity for adenosine triphosphate. E. Coli LPS did not potentiate the effect of parathyroid hormone on glomerular and tubular adenylate cyclase. The lipid A moiety which is common to all LPS whatever the original strain gave results similar to those obtained with the entire LPS. This effect was specific and did not depend on the phospholipidic structure in general since no activation was obtained in the presence of phosphatidylserine.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1977
          1977
          02 December 2008
          : 19
          : 6
          : 342-349
          Affiliations
          Unité de Recherches de Néphrologie Normale et Pathologique de l’lnserm U-64, Hôpital Tenon, Paris
          Article
          180912 Nephron 1977;19:342–349
          10.1159/000180912
          337175
          © 1977 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

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