- Record: found
- Abstract: not found
- Article: not found
Universal sample preparation method for proteome analysis
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
We describe a method, filter-aided sample preparation (FASP), which combines the advantages
of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely
solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea
on a standard filtration device. Peptides eluted after digestion on the filter were
pure, allowing single-run analyses of organelles and an unprecedented depth of proteome
coverage.