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      Universal sample preparation method for proteome analysis.

      Nature Methods

      methods, Animals, Brain Chemistry, Endopeptidases, chemistry, HeLa Cells, Humans, Iodoacetamide, Liver, Mass Spectrometry, Mice, Mitochondria, Proteome, analysis, Proteomics, Sodium Dodecyl Sulfate, Tandem Mass Spectrometry, Ultrafiltration, Urea, Analytic Sample Preparation Methods

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          Abstract

          We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

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          Journal
          19377485
          10.1038/nmeth.1322

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