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      Universal sample preparation method for proteome analysis

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          Abstract

          We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.

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          Author and article information

          Journal
          Nature Methods
          Nat Methods
          Springer Science and Business Media LLC
          1548-7091
          1548-7105
          May 2009
          April 19 2009
          May 2009
          : 6
          : 5
          : 359-362
          Article
          10.1038/nmeth.1322
          19377485
          d04dad08-0ce6-4fa0-a3c5-0fc471c23f5c
          © 2009

          http://www.springer.com/tdm

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