This paper presents the results of the performance test and long-term monitoring of the prestressing force inside concrete performed on a pretensioned Ultra-High Performance Concrete (UHPC) deck. The force is measured by applying a 7-wire strand embedded with an FBG (Fiber Bragg Grating) sensor. The performance test was conducted on a 3.7 m × 1.8 m pretensioned deck specimen through wheel loading tests to verify the applicability of the measurement method. In addition, a 12.3 m long and 4.8 m wide bridge with a pretensioned UHPC deck was erected and long-term monitoring was conducted over three years to verify the applicability of the method to real bridges. The effectiveness of the measurement method of the prestressing force inside concrete is verified, and the long-term monitoring data are used to investigate various temperature compensation methods. The results show that the proposed method enables effective measurement of small changes in the prestressing force inside the concrete. These changes are caused by the external forces acting on the bridge in service and provide sufficient durability for long-term sensing. The analysis of the prestressing force obtained through long-term monitoring reveals the necessity of conducting temperature compensation for the consistency of the data acquired using the FBG sensor. Moreover, the selection of the thermal expansion coefficient appears also to be of critical importance for temperature compensation.