Blog
About

  • Record: found
  • Abstract: not found
  • Article: not found

Array-based “Chemical Nose” Sensing in Diagnostics and Drug Discovery

Read this article at

ScienceOpenPublisher
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Related collections

      Most cited references 112

      • Record: found
      • Abstract: not found
      • Article: not found

      Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.

        Bookmark
        • Record: found
        • Abstract: not found
        • Article: not found

        Gold nanoparticles in chemical and biological sensing.

          Bookmark
          • Record: found
          • Abstract: found
          • Article: not found

          Using Fourier transform IR spectroscopy to analyze biological materials.

          IR spectroscopy is an excellent method for biological analyses. It enables the nonperturbative, label-free extraction of biochemical information and images toward diagnosis and the assessment of cell functionality. Although not strictly microscopy in the conventional sense, it allows the construction of images of tissue or cell architecture by the passing of spectral data through a variety of computational algorithms. Because such images are constructed from fingerprint spectra, the notion is that they can be an objective reflection of the underlying health status of the analyzed sample. One of the major difficulties in the field has been determining a consensus on spectral pre-processing and data analysis. This manuscript brings together as coauthors some of the leaders in this field to allow the standardization of methods and procedures for adapting a multistage approach to a methodology that can be applied to a variety of cell biological questions or used within a clinical setting for disease screening or diagnosis. We describe a protocol for collecting IR spectra and images from biological samples (e.g., fixed cytology and tissue sections, live cells or biofluids) that assesses the instrumental options available, appropriate sample preparation, different sampling modes as well as important advances in spectral data acquisition. After acquisition, data processing consists of a sequence of steps including quality control, spectral pre-processing, feature extraction and classification of the supervised or unsupervised type. A typical experiment can be completed and analyzed within hours. Example results are presented on the use of IR spectra combined with multivariate data processing.
            Bookmark

            Author and article information

            Affiliations
            [1 ]Molecular and Cellular Biology Program; University of Massachusetts Amherst; 710 N. Pleasant St. Amherst MA 01003 USA
            [2 ]Department of Chemistry; University of Massachusetts Amherst; 710 N. Pleasant St. Amherst MA 01003 USA
            [3 ]Division of Biomedical Engineering; School of Engineering; University of Glasgow; Glasgow G12 8LT UK
            [4 ]Department of Chemistry; University of British Columbia; 2036 Main Mall Vancouver British Columbia V6T 1Z1 Canada
            Journal
            Angewandte Chemie International Edition
            Angew. Chem. Int. Ed.
            Wiley
            14337851
            April 08 2019
            April 08 2019
            February 20 2019
            : 58
            : 16
            : 5190-5200
            10.1002/anie.201809607
            © 2019

            http://doi.wiley.com/10.1002/tdm_license_1.1

            http://onlinelibrary.wiley.com/termsAndConditions#vor

            Comments

            Comment on this article