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      The effects of cytochalasins on the ultrastructure of neurulating hamster embryos in vivo.

      Teratology
      Wiley-Blackwell

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          Abstract

          Single intraperitoneal injections of cytochalasin B (CB) in dimethylsulfoxide were given to gravid Syrian hamsters on the eighth day of pregnancy at various dose levels. Exencephaly and encephalocele, the only defects which were seen in the term litters, occurred in dose-response patterns reaching peak frequencies of 14.9% and 53.2%, respectively, at the highest dose level, while accompanied by a mortality of 27.7% of implantations. Although these abnormalities were the same as those resulting from cytochalasin D (CD) treatment at this time, the frequencies were lower and the distribution of defects somewhat different. Morphological comparison of embryos fixed at various times after maternal treatment with 7.0 mg/kg CB or 1.5 mg/kg CD demonstrated qualitatively similar changes in response to either teratogen, leading to failure of the cranial neural folds to approximate and close. The principal ultrastructural changes involved alterations in the topography of the apical membranes of neuroectoderm cells. At doses which produced high frequencies of gross defects in the term litters, no changes were seen in the apical bundles of microfilaments in these cells, although much higher dose levels did disrupt these structures. The results support the hypothesis that the cell membrane is the primary target of these teratogens in vivo.

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          Author and article information

          Journal
          Teratology
          Teratology
          Wiley-Blackwell
          0040-3709
          0040-3709
          Aug 1980
          : 22
          : 1
          Article
          10.1002/tera.1420220109
          7444801
          d0c64988-0757-4553-85e5-22cfe0f318be
          History

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