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      Possible Involvement of Lipoxygenase Products in Human Corpora lutea

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          Abstract

          The present study was undertaken to determine the ability of cultured luteal cells from human corpora lutea to secrete progesterone (P<sub>4</sub>) and prostaglandins (PGs), and to assess the effects of the products of the lipoxygenase pathway on luteal P<sub>4</sub> production. Luteal cells responded to human chorionic gonadotropin (hCG) with a significant increase (2- to 7-fold) in P<sub>4</sub> production. Arachidonic acid significantly stimulated PGE<sub>2</sub> synthesis by luteal cells in a dose-dependent manner. Both basal PGE<sub>2</sub> production and the responsiveness to arachidonic acid were maintained for 8 days. In contrast, both PGF<sub>2α</sub> and 6-keto-PGF<sub>1α</sub> production abruptly declined as the culture proceeded. However, the addition of hCG did not further stimulate the accumulation of the 3 PGs assayed. In the subsequent experiment, 5-hydroxyeicosatetraenoic acid (5-HETE) and the reaction products of soybean lipoxidase of arachidonic acid (AA-LIP) were utilized for evaluating the involvement of the lipoxygenase pathway in luteolysis. The addition of 5-HETE dose-dependently inhibited P<sub>4</sub> production by the cultured luteal cells. Although treatment with either arachidonic acid or lipoxidase alone had no effect on P<sub>4</sub> production, AA-LIP significantly reduced P<sub>4</sub> production in the presence or absence of hCG. These results suggest that the products of the lipoxygenase as well as of the cyclo-oxygenase pathway may be important in regulating the life span and function of human corpora lutea.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          978-3-8055-5625-5
          978-3-318-01972-8
          1663-2818
          1663-2826
          1992
          1992
          03 December 2008
          : 37
          : Suppl 1
          : 19-24
          Affiliations
          aDepartment of Obstetrics and Gynecology, Kyorin University School of Medicine, Tokyo; bDepartment of Obstetrics and Gynecology, Tokyo Dental College, Ichikawa General Hospital, Ichikawa. Chiba. Japan
          Article
          182340 Horm Res 1992;37:19–24
          10.1159/000182340
          1427623
          © 1992 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 6
          Categories
          New Aspects of the Physiology and Pathology of the Luteal Phase

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