Blog
About

6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Effect of preservation method on spider monkey (Ateles geoffroyi) fecal microbiota over 8 weeks.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Studies of the gut microbiome have become increasingly common with recent technological advances. Gut microbes play an important role in human and animal health, and gut microbiome analysis holds great potential for evaluating health in wildlife, as microbiota can be assessed from non-invasively collected fecal samples. However, many common fecal preservation protocols (e.g. freezing at -80 °C) are not suitable for field conditions, or have not been tested for long-term (greater than 2 weeks) storage. In this study, we collected fresh fecal samples from captive spider monkeys (Ateles geoffroyi) at the Columbian Park Zoo (Lafayette, IN, USA). The samples were pooled, homogenized, and preserved for up to 8 weeks prior to DNA extraction and sequencing. Preservation methods included: freezing at -20 °C, freezing at -80 °C, immersion in 100% ethanol, application to FTA cards, and immersion in RNAlater. At 0 (fresh), 1, 2, 4, and 8 weeks from fecal collection, DNA was extracted and microbial DNA was amplified and sequenced. DNA concentration, purity, microbial diversity, and microbial composition were compared across all methods and time points. DNA concentration and purity did not correlate with microbial diversity or composition. Microbial composition of frozen and ethanol samples were most similar to fresh samples. FTA card and RNAlater-preserved samples had the least similar microbial composition and abundance compared to fresh samples. Microbial composition and diversity were relatively stable over time within each preservation method. Based on these results, if freezers are not available, we recommend preserving fecal samples in ethanol (for up to 8weeks) prior to microbial extraction and analysis.

          Related collections

          Author and article information

          Journal
          J. Microbiol. Methods
          Journal of microbiological methods
          1872-8359
          0167-7012
          Jun 2015
          : 113
          Affiliations
          [1 ] Department of Biological Sciences, Purdue University, West Lafayette, IN 47906, USA. Electronic address: hale.vanessa@mayo.edu.
          [2 ] San Diego Zoo Institute for Conservation Research, Escondido, CA 92027, USA. Electronic address: ctan@sandiegozoo.org.
          [3 ] Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA; Department of Computer Science & Engineering, University of California San Diego, La Jolla, California 92093, USA. Electronic address: robknight@ucsd.edu.
          [4 ] Department of Anthropology, University of Colorado, Boulder, CO 80309, USA. Electronic address: Katherine.Amato@colorado.edu.
          Article
          S0167-7012(15)00110-4
          10.1016/j.mimet.2015.03.021
          25819008
          Copyright © 2015 Elsevier B.V. All rights reserved.

          Comments

          Comment on this article