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      Novel Reassortant Avian Influenza A(H3N8) Virus Isolated from a Wild Bird in Jiangxi, China

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          Abstract

          Here, we report the detection of a reassortant avian influenza A(H3N8) virus isolated from a wild bird in Poyang Lake, Jiangxi, China, in 2014. Phylogenetic analyses indicated that this virus is most likely derived from the Eurasian-origin H3Ny and HxN8 viruses and two strains endemic to China, namely, H5N1 and H5N6.

          ABSTRACT

          Here, we report the detection of a reassortant avian influenza A(H3N8) virus isolated from a wild bird in Poyang Lake, Jiangxi, China, in 2014. Phylogenetic analyses indicated that this virus is most likely derived from the Eurasian-origin H3Ny and HxN8 viruses and two strains endemic to China, namely, H5N1 and H5N6.

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          Identification and subtyping of avian influenza viruses by reverse transcription-PCR.

          Ming-Shiuh Lee, Poa-Chun Chang, Jui-Hung Shien … (2001)
          Avian influenza viruses have 15 different hemagglutinin (HA) subtypes (H1-H15). We report a procedure for the identification and HA-subtyping of avian influenza virus by reverse transcription-PCR (RT-PCR). The avian influenza virus is identified by RT-PCR using a set of primers specific to the nucleoprotein (NP) gene of avian influenza virus. The HA-subtypes of avian influenza virus were determined by running simultaneously 15 RT-PCR reactions, each using a set of primers specific to one HA-subtype. For a single virus strain or isolate, only one of the 15 RT-PCR reactions will give a product of expected size, and thus the HA-subtype of the virus is determined. The result of HA-subtyping was then confirmed by sequence analysis of the PCR product. A total of 80 strains or isolates of avian influenza viruses were subtyped by this RT-PCR procedure, and the result of RT-PCR gave an excellent (100%) correlation with the result of the conventional serological method. The RT-PCR procedure we developed is rapid and sensitive, and could be used for the identification and HA-subtyping of avian influenza virus in organ homogenates.
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            Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks at the Dongting Lake Region of China.

            Guohua Deng, Liling Liu, Yoshihiro Kawaoka … (2013)
            To gain insight into the ecology of avian influenza viruses (AIV), we conducted active influenza virus surveillance in domestic ducks on farms located on the flyway of migratory birds in the Dongting Lake region of Hunan Province, China, from winter 2011 until spring 2012. Specimens comprising 3,030 duck swab samples and 1,010 environmental samples were collected from 101 duck farms. We isolated AIV of various HA subtypes, including H3, H4, H5, H6, H9, H10, H11, and H12. We sequenced the entire coding sequences of the genomes of 28 representative isolates constituting 13 specific subtypes. When the phylogenetic relationships among these isolates were examined, we observed that extensive reassortment events had occurred. Among the 28 Dongting Lake viruses, 21 genotypes involving the six internal genes were identified. Furthermore, we identified viruses or viral genes introduced from other countries, viral gene segments of unknown origin, and a novel HA/NA combination. Our findings emphasize the importance of farmed domestic ducks in the Dongting Lake region to the genesis and evolution of AIV and highlight the need for continued surveillance of domestic ducks in this region.
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              Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.

              T Ashizawa, Masatoshi Okamatsu, Kotaro Suzuki … (2009)
              Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
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                Author and article information

                Contributors
                Simon Roux: Role: Editor
                Journal
                Journal ID (nlm-ta): Microbiol Resour Announc
                Journal ID (iso-abbrev): Microbiol Resour Announc
                Journal ID (hwp): ga
                Journal ID (pmc): mra
                Journal ID (publisher-id): MRA
                Title: Microbiology Resource Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2576-098X
                Publication date (Electronic): 14 November 2019
                Publication date Collection: November 2019
                Volume: 8
                Issue: 46
                Electronic Location Identifier: e01163-19
                Affiliations
                [a ]MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine, Imperial College London, London, United Kingdom
                [b ]Ministry of Education Key Laboratory for Earth System Modelling, Department of Earth System Science, Tsinghua University, Beijing, China
                [c ]Centre for Healthy Cities, Institute for China Sustainable Urbanization, Tsinghua University, Beijing, China
                [d ]School of Geography and Environmental Science, Ministry of Education Key Laboratory of Poyang Lake Wetland and Watershed Research, Jiangxi Normal University, Nanchang, Jiangxi, China
                [e ]College of Veterinary Medicine, China Agricultural University, Beijing, China
                DOE Joint Genome Institute
                Author notes
                Address correspondence to Jianyu Chang, changjianyu@ 123456cau.edu.cn , or Bing Xu, bingxu@ 123456tsinghua.edu.cn .

                R.L. and T.Z. contributed equally to this work.

                Citation Li R, Zhang T, Xu J, Chang J, Xu B. 2019. Novel reassortant avian influenza A(H3N8) virus isolated from a wild bird in Jiangxi, China. Microbiol Resour Announc 8:e01163-19. https://doi.org/10.1128/MRA.01163-19.

                Author information
                https://orcid.org/0000-0001-8927-9965
                Article
                Publisher ID: MRA01163-19
                DOI: 10.1128/MRA.01163-19
                PMC ID: 6856275
                PubMed ID: 31727709
                SO-VID: d15e51ac-2eaa-4424-9a94-f4fe15078ecf
                Copyright © Copyright © 2019 Li et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 9 October 2019
                Date accepted : 23 October 2019
                Page count
                Figures: 1, Tables: 1, Equations: 0, References: 9, Pages: 3, Words: 1719
                Funding
                Funded by: Ministry of Science and Technology of the People's Republic of China (MOST), https://doi.org/10.13039/501100002855;
                Award ID: 2016YFA0600104
                Award Recipient :
                Categories
                Subject: Genome Sequences
                Custom metadata
                cover-date November 2019

                Data availability:

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