Lúcio Ricardo Leite Diniz 1 , Viviane Gomes Portella 2 , Kerly Shamira da Silva Alves 2 , Pâmella Cristina da Costa Araújo 2 , Ricardo Luiz Cavalcanti de Albuquerque Júnior 3 , Aline Alice Cavalcante de Albuquerque 2 , Andrelina Noronha Coelho-de-Souza 2 , José Henrique Leal-Cardoso 2
26 April 2018
Nonspecific and delayed diagnosis of neurologic damage contributes to the development of neuropathies in patients with severe sepsis. The present study assessed the electrophysiologic parameters related to the excitability and conductibility of sciatic and vagus nerves during early stages of sepsis.
Twenty-four hours after sepsis induced by cecal ligation and puncture (CLP) model, sciatic and vagus nerves of septic (CLP group) and control (sham group) rats were removed, and selected electric stimulations were applied to measure the parameters of the first and second components of the compound action potential. The first component originated from fibers with motor and sensory functions (Types A α and A β fibers) with a large conduction velocity (70–120 m/s), and the second component originated from fibers (Type A γ) with sensorial function. To evaluate the presence of sensorial alterations, the sensitivity to non-noxious mechanical stimuli was measured by using the von Frey test. Hematoxylin and eosin staining of the nerves was performed.
We observed an increase of rheobase followed by a decrease in the first component amplitude and a higher paw withdrawal threshold in response to the application of von Frey filaments in sciatic nerves from the CLP group compared to the sham group. Differently, a decrease in rheobase and an increase in the first component amplitude of vagal C fibers from CLP group were registered. No significant morphologic alteration was observed.