Study of Awakening Rabbit Cerebral Dormancy by Perfusion

Abstract

Objective: To explore the mechanism of awakening rabbit cerebral dormancy by perfusion and its relationship with brain death.

Methods: The study selected 36 healthy New Zealand white rabbits from september 2010 to 2014. Using random number table method, we divided them into 6 groups (group A-F) with 6 rabbits in each group. Controllable whole brain ischemia experiment models were built, and comparison was made among the awakening effects of conventional resuscitation, self-formulated rabbit cerebral protective fluid, 5% glucose solution and rabbit plasma. By MS-2000 computer biological signal system, we recorded and analyzed breath, heart rate, blood pressure and electroencephalogram of the rabbits during the whole intervention. Autokinetic movement, response to stimulation and brain edema were observed. The survival rates of the 6 groups were recorded. We selected 6 healthy New Zealand white rabbits and obtained their anticoagulant plasma and tissue extracts of brain, liver, heart and kidney. Using tube method, we compared the influences of brain, liver, heart and kidney extracts on blood coagulation time.

Results: The rabbits of group A, group C and group D all died because of the irreparability of autonomous respiration; the rabbits of group B all recovered reflection and survived; 1 rabbit in group E died because of the irreparability of autonomous respiration, and the rest 5 rabbits recovered reflection and survived; 3 rabbits in group F died due to the irreparability of autonomous respiration, and the rest 3 rabbits recovered reflection and survived. The 6 groups were significantly different in survival rate (P＜0.05). Group B, group E were higher than group A, group C, group D in survival rate (P＜0.05). The five tubes of blood were significantly different in coagulation time (P＜0.05). Tube 2, tube 3, tube 4 and tube 5 had longer blood coagulation time than tube 1 (P＜0.05); tube 3, tube 4 and tube 5 had longer blood coagulation time than tube 2 (P＜0.05), tube 4 had shorter blood coagulation time than tube 3 (P＜0.05), and tube 5 had longer blood coagulation time than tube 3 (P＜0.05); tube 5 had longer blood coagulation time than tube 4 (P＜0.05).

Conclusion: The self-developed cerebral protective fluid can effectively prevent cerebral edema and blood coagulation that occur rapidly after the halt of cerebral blood supply, with the cerebral dormancy prolonging 60 minutes. The study may provide references for further clinical research.