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      Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.

      1 , ,
      Nature
      Springer Science and Business Media LLC

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          Abstract

          Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

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          Author and article information

          Journal
          Nature
          Nature
          Springer Science and Business Media LLC
          0028-0836
          0028-0836
          Nov 24 1988
          : 336
          : 6197
          Affiliations
          [1 ] Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.
          Article
          10.1038/336348a0
          3194019
          d26d15dc-ab97-44e3-b95e-d27073be23c6
          History

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