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      Advancement in POCT Molecular Testing: The Multiplex PCR POCT Devices for Infectious Diseases

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          Abstract

          Rapid and accurate diagnostic tests are very important for the global control of infectious diseases. The point of care diagnosis has become a promising strategy in recent years. Different kind of point of care testing devices has been introduced into the market in the last decade. These devices must provide a low-cost, robust, sensitive, specific, and practical analysis in order to replace the conventional clinical laboratory diagnostic test algorithms when needed. The successful implementation of point of care diagnostics has a potential to increase the strength of infectious diseases surveillance programs. Finally, the rapid progress in point of care diagnosis can stimulate a shift from a centralized diagnostic model to a decentralized patient-centered approach.

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          Most cited references19

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          The present and future role of microfluidics in biomedical research.

          Microfluidics, a technology characterized by the engineered manipulation of fluids at the submillimetre scale, has shown considerable promise for improving diagnostics and biology research. Certain properties of microfluidic technologies, such as rapid sample processing and the precise control of fluids in an assay, have made them attractive candidates to replace traditional experimental approaches. Here we analyse the progress made by lab-on-a-chip microtechnologies in recent years, and discuss the clinical and research areas in which they have made the greatest impact. We also suggest directions that biologists, engineers and clinicians can take to help this technology live up to its potential.
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            Three-dimensional microfluidic devices fabricated in layered paper and tape.

            This article describes a method for fabricating 3D microfluidic devices by stacking layers of patterned paper and double-sided adhesive tape. Paper-based 3D microfluidic devices have capabilities in microfluidics that are difficult to achieve using conventional open-channel microsystems made from glass or polymers. In particular, 3D paper-based devices wick fluids and distribute microliter volumes of samples from single inlet points into arrays of detection zones (with numbers up to thousands). This capability makes it possible to carry out a range of new analytical protocols simply and inexpensively (all on a piece of paper) without external pumps. We demonstrate a prototype 3D device that tests 4 different samples for up to 4 different analytes and displays the results of the assays in a side-by-side configuration for easy comparison. Three-dimensional paper-based microfluidic devices are especially appropriate for use in distributed healthcare in the developing world and in environmental monitoring and water analysis.
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              Point of Care Testing for Infectious Diseases -- Past, Present and Future.

              Point-of-care (POC) diagnostics provide rapid actionable information for patient care at the time and site of encounter with the health care system. The usual platform has been the lateral flow immunoassay. Recently, emerging molecular diagnostics have met requirements for speed, low cost and ease of use for POC applications. A major driver for POC development is the ability to diagnose infectious diseases at sites with limited infrastructure. Potential use in both wealthy and resource-limited settings has fueled an intense effort to build on existing technologies and to generate new technologies for diagnosis of a broad spectrum of infectious diseases.
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                Author and article information

                Journal
                EJIFCC
                EJIFCC
                eJIFCC
                EJIFCC
                The Communications and Publications Division (CPD) of the IFCC
                1650-3414
                07 November 2018
                November 2018
                : 29
                : 3
                : 205-209
                Affiliations
                Department of Medical Microbiology, Faculty of Medicine, Hacettepe University , Ankara, Turkey
                Author notes
                Corresponding author: Alpaslan Alp Department of Medical Microbiology Faculty of Medicine Hacettepe University Ankara Turkey E-mail: alp1086@ 123456gmail.com
                Article
                ejifcc-29-205
                6247132
                30479605
                d280e6bc-4ecd-4f8f-babe-3c68fb8bd67c
                Copyright © 2018 International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). All rights reserved.

                This is a Platinum Open Access Journal distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 20, Pages: 5
                Categories
                Discussion

                point of care test,multiplex point of care device,microfluidics,lab-on-a-chip,infectious disease

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