3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.

          Abstract

          Nonstructural protein 15 (nsp15), also called endoribonuclease, is a gene product of open reading frame 1b (ORF 1b) in coronaviruses. It is an important enzyme in the transcription/replication process involved in discontinuous negative-strand RNA synthesis. In this work, mutants of nsp15 from Human coronavirus 229E (HCoV-229E) were made based on structural analysis of the homologous nsp15s in Severe acute respiratory syndrome coronavirus (SARS-CoV) and Mouse hepatitis virus (MHV). The I26A/N52A mutant of nsp15 was overexpressed, purified and crystallized, and this mutant led to a trimeric form rather than hexamers or monomers. Crystals of trimeric nsp15 were obtained by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant and diffracted to 2.5 Å resolution. The crystals belonged to space group C222 1, with unit-cell parameters a = 85.9, b = 137.5, c = 423.1 Å, α = β = γ = 90°.

          Related collections

          Author and article information

          Journal
          Acta Crystallogr F Struct Biol Commun
          Acta Crystallogr F Struct Biol Commun
          Acta Cryst. F
          Acta Crystallographica. Section F, Structural Biology Communications
          International Union of Crystallography
          2053-230X
          1 September 2015
          25 August 2015
          1 September 2017
          : 71
          : Pt 9 ( publisher-idID: f150900 )
          : 1156-1160
          Affiliations
          [a ]College of Life Sciences, Nankai University , Tianjin 300071, People’s Republic of China
          [b ]High-Throughput Molecular Drug Discovery Center, Tianjin Joint Academy of Biomedicine and Technology , Tianjin 300457, People’s Republic of China
          Author notes
          Correspondence e-mail: cameliunx@ 123456163.com
          Article
          hc5195 ACSFEN S2053230X15007359
          10.1107/S2053230X15007359
          4555923
          26323302
          d2b2c5a2-8e76-4a84-b5e6-b43b0f55bf7c
          © International Union of Crystallography 2015
          History
          : 26 December 2014
          : 14 April 2015
          Categories
          Research Communications

          coronaviruses,human coronavirus 229e,nonstructural protein 15,endoribonuclease,trimeric form

          Comments

          Comment on this article