Now-a-days, top-down proteomics (TDP) is a booming approach for the analysis of intact
proteins and it is attaining significant interest in the field of protein biology.
The term has emerged as an alternative to the well-established, bottom-up strategies
for analysis of peptide fragments derived from either enzymatically or chemically
digestion of intact proteins. TDP is applied to mass spectrometric analysis of intact
large biomolecules that are constituents of protein complexes and assemblies. This
article delivers an overview of the methodologies in top-down mass spectrometry, mass
spectrometry instrumentation and an extensive review of applications covering the
venomics, biomedical research, protein biology including the analysis of protein post-translational
modifications (PTMs), protein biophysics, and protein complexes. In addition, limitations
of top-down proteomics, challenges and future directions of TDP are also discussed.