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      Top-down proteomics: applications, recent developments and perspectives

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      Journal of Applied Bioanalysis

      Betasciencepress Publishing

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          Abstract

          Now-a-days, top-down proteomics (TDP) is a booming approach for the analysis of intact proteins and it is attaining significant interest in the field of protein biology. The term has emerged as an alternative to the well-established, bottom-up strategies for analysis of peptide fragments derived from either enzymatically or chemically digestion of intact proteins. TDP is applied to mass spectrometric analysis of intact large biomolecules that are constituents of protein complexes and assemblies. This article delivers an overview of the methodologies in top-down mass spectrometry, mass spectrometry instrumentation and an extensive review of applications covering the venomics, biomedical research, protein biology including the analysis of protein post-translational modifications (PTMs), protein biophysics, and protein complexes. In addition, limitations of top-down proteomics, challenges and future directions of TDP are also discussed.

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          Most cited references 7

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          Electrospray ionization for mass spectrometry of large biomolecules

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            Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons

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              Protein and polymer analyses up tom/z 100 000 by laser ionization time-of-flight mass spectrometry

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                Author and article information

                Journal
                Journal of Applied Bioanalysis
                J Appl Bioanal
                Betasciencepress Publishing
                2405710X
                April 13 2016
                April 13 2016
                : 2
                : 2
                : 52-75
                Article
                10.17145/jab.16.009
                © 2016

                This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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                Self URI (journal-page): https://jab.scholasticahq.com/

                General life sciences, Chemistry, Analytical chemistry, Life sciences

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