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Top-down proteomics: applications, recent developments and perspectives

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Journal of Applied Bioanalysis

Betasciencepress Publishing

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      Abstract

      Now-a-days, top-down proteomics (TDP) is a booming approach for the analysis of intact proteins and it is attaining significant interest in the field of protein biology. The term has emerged as an alternative to the well-established, bottom-up strategies for analysis of peptide fragments derived from either enzymatically or chemically digestion of intact proteins. TDP is applied to mass spectrometric analysis of intact large biomolecules that are constituents of protein complexes and assemblies. This article delivers an overview of the methodologies in top-down mass spectrometry, mass spectrometry instrumentation and an extensive review of applications covering the venomics, biomedical research, protein biology including the analysis of protein post-translational modifications (PTMs), protein biophysics, and protein complexes. In addition, limitations of top-down proteomics, challenges and future directions of TDP are also discussed.

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      Most cited references 7

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      Electrospray ionization for mass spectrometry of large biomolecules

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        Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons

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          Protein and polymer analyses up tom/z 100 000 by laser ionization time-of-flight mass spectrometry

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            Author and article information

            Journal
            Journal of Applied Bioanalysis
            J Appl Bioanal
            Betasciencepress Publishing
            2405710X
            April 13 2016
            April 13 2016
            : 2
            : 2
            : 52-75
            10.17145/jab.16.009
            © 2016

            This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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            Self URI (journal-page): https://jab.scholasticahq.com/

            General life sciences, Chemistry, Analytical chemistry, Life sciences

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