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      FSOCA-induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo.

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          Abstract

          The mouse footpad for its feature of hairlessness provides an available window for imaging vascular and cellular structure and function in vivo. Unfortunately, the strong scattering of its skin limits the penetration of light and reduces the imaging contrast and depth. Herein, an innovative footpad skin optical clearing agent (FSOCA) was developed to make the footpad skin transparent quickly by topical application. The results demonstrate that FSOCA treatment not only allowed the cutaneous blood vessels and blood flow distribution to be monitored by laser speckle contrast imaging technique with higher contrast, but also permitted the fluorescent cells to be imaged by laser scanning confocal microscopy with higher fluorescence signal intensity and larger imaging depth. In addition, the physiological saline-treatment could make the footpad skin recover to the initial turbid status, and reclearing would not induce any adverse effects on the distributions and morphologies of blood vessels and cells, which demonstrated a safe and switchable window for biomedical imaging. This switchable footpad skin optical clearing window will be significant for studying blood flow dynamics and cellular immune function in vivo in some vascular and immunological diseases. Picture: Repeated cell imaging in vivo before (a) and after (b) FSOCA treatment. (c) Merged images of 4 h (cyan border) or 72 h (magenta border) over 0 h. (d) Zoom of ROI in 4 h (yellow rectangle) or 72 h (red rectangle).

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          Author and article information

          Journal
          J Biophotonics
          Journal of biophotonics
          Wiley-Blackwell
          1864-0648
          1864-063X
          May 18 2017
          Affiliations
          [1 ] Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics (WNLO), Huazhong University of Science and Technology (HUST), 1037 Luoyu Road, Wuhan, 430074, Hubei, P. R. China.
          [2 ] Key Laboratory of Biomedical Photonics, HUST, Ministry of Education, 1037 Luoyu Road, Wuhan, 430074, Hubei, P. R. China.
          [3 ] Hubei Bioinformatics and Bioimaging Key Laboratory, Department of Biomedical Engineering, HUST, 1037 Luoyu Road, Wuhan, 430074, Hubei, P. R. China.
          Article
          10.1002/jbio.201700052
          28516571
          d30c1e09-796e-4e14-ab61-1756b3f1992e
          History

          blood flow,cell,confocal microscopy,footpad skin optical clearing,laser speckle contrast imaging

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