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      Cabergoline, Dopamine D2 Receptor Agonist, Prevents Neuronal Cell Death under Oxidative Stress via Reducing Excitotoxicity

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          Abstract

          Several lines of evidence demonstrate that oxidative stress is involved in the pathogenesis of neurodegenerative diseases, including Parkinson's disease. Potent antioxidants may therefore be effective in the treatment of such diseases. Cabergoline, a dopamine D2 receptor agonist and antiparkinson drug, has been studied using several cell types including mesencephalic neurons, and is recognized as a potent radical scavenger. Here, we examined whether cabergoline exerts neuroprotective effects against oxidative stress through a receptor-mediated mechanism in cultured cortical neurons. We found that neuronal death induced by H 2O 2 exposure was inhibited by pretreatment with cabergoline, while this protective effect was eliminated in the presence of a dopamine D 2 receptor inhibitor, spiperone. Activation of ERK1/2 by H 2O 2 was suppressed by cabergoline, and an ERK signaling pathway inhibitor, U0126, similarly protected cortical neurons from cell death. This suggested the ERK signaling pathway has a critical role in cabergoline-mediated neuroprotection. Furthermore, increased extracellular levels of glutamate induced by H 2O 2, which might contribute to ERK activation, were reduced by cabergoline, while inhibitors for NMDA receptor or L-type Ca 2+ channel demonstrated a survival effect against H 2O 2. Interestingly, we found that cabergoline increased expression levels of glutamate transporters such as EAAC1. Taken together, these results suggest that cabergoline has a protective effect on cortical neurons via a receptor-mediated mechanism including repression of ERK1/2 activation and extracellular glutamate accumulation induced by H 2O 2.

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          Most cited references 46

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          Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.

          Multicellular organisms have three well-characterized subfamilies of mitogen-activated protein kinases (MAPKs) that control a vast array of physiological processes. These enzymes are regulated by a characteristic phosphorelay system in which a series of three protein kinases phosphorylate and activate one another. The extracellular signal-regulated kinases (ERKs) function in the control of cell division, and inhibitors of these enzymes are being explored as anticancer agents. The c-Jun amino-terminal kinases (JNKs) are critical regulators of transcription, and JNK inhibitors may be effective in control of rheumatoid arthritis. The p38 MAPKs are activated by inflammatory cytokines and environmental stresses and may contribute to diseases like asthma and autoimmunity.
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            ERK and cell death: mechanisms of ERK-induced cell death--apoptosis, autophagy and senescence.

            The Ras/Raf/extracellular signal-regulated kinase (ERK) signaling pathway plays a crucial role in almost all cell functions and therefore requires exquisite control of its spatiotemporal activity. Depending on the cell type and stimulus, ERK activity will mediate different antiproliferative events, such as apoptosis, autophagy and senescence in vitro and in vivo. ERK activity can promote either intrinsic or extrinsic apoptotic pathways by induction of mitochondrial cytochrome c release or caspase-8 activation, permanent cell cycle arrest or autophagic vacuolization. These unusual effects require sustained ERK activity in specific subcellular compartments and could depend on the presence of reactive oxygen species. We will summarize the mechanisms involved in Ras/Raf/ERK antiproliferative functions.
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              Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate.

              Three glutamate transporters have been identified in rat, including astroglial transporters GLAST and GLT-1 and a neuronal transporter EAAC1. Here we demonstrate that inhibition of the synthesis of each glutamate transporter subtype using chronic antisense oligonucleotide administration, in vitro and in vivo, selectively and specifically reduced the protein expression and function of glutamate transporters. The loss of glial glutamate transporters GLAST or GLT-1 produced elevated extracellular glutamate levels, neurodegeneration characteristic of excitotoxicity, and a progressive paralysis. The loss of the neuronal glutamate transporter EAAC1 did not elevate extracellular glutamate in the striatum but did produce mild neurotoxicity and resulted in epilepsy. These studies suggest that glial glutamate transporters provide the majority of functional glutamate transport and are essential for maintaining low extracellular glutamate and for preventing chronic glutamate neurotoxicity.
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                Author and article information

                Affiliations
                [1 ]Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan
                [2 ]Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
                [3 ]Core Research for Evolution Science and Technology Program (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan
                Universidad de Castilla-La Mancha, Spain
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Conceived and designed the experiments: HO TN NA TI HK. Performed the experiments: HO TN YK NA. Analyzed the data: HO NA YO SN YK. Contributed reagents/materials/analysis tools: TN HK. Wrote the paper: HO TN.

                Contributors
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, USA )
                1932-6203
                2014
                10 June 2014
                : 9
                : 6
                PONE-D-13-47278
                10.1371/journal.pone.0099271
                4051758
                24914776

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                Counts
                Pages: 12
                Funding
                This study was supported by the Intramural Research Grant (No. 24-11) for Neurological and Psychiatric Disorders of NCNP ( http://www.ncnp.go.jp/) and CREST program “creation of a novel technology towards diagnosis and treatment based on understanding of molecular pathogenesis of psychiatric and neurological disorders” (No. 08062571) of JST ( http://www.sss.jst.go.jp/info/index.html) (T.N., N.A. and H.K.). This project was also supported by Takeda Science Foundation ( http://www.takeda-sci.or.jp/) (T.N.), a grant from Grant-in-Aid for Scientific Research (B) (JSPS KAKENHI Grant Number 24300139) ( http://www.jsps.go.jp/english/index.html) (T.N.), and Grant-in-Aid for Challenging Exploratory Research (JSPS KAKENHI Grant Number 25640019) ( http://www.jsps.go.jp/english/index.html) (T.N.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Biology and life sciences
                Biochemistry
                Hormones
                Catecholamines
                Dopamine
                Neurochemistry
                Neurochemicals
                Cell biology
                Cell Processes
                Cell Death
                Cellular Types
                Animal Cells
                Neurons
                Signal transduction
                Cell signaling
                Signaling cascades
                ERK signaling cascade
                Molecular Cell Biology
                Toxicology
                Neurotoxicology
                Medicine and Health Sciences
                Clinical Medicine
                Pharmacology
                Drug Research and Development

                Uncategorized

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