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      Role of stellate cells in hepatic echinococcosis in cattle

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          Abstract

          Hydatid cysts formed by the metacestodes of Echinococcus granulosus. Cattle suffering from hydatid cyst shows fluid-filled structures, especially in liver. These parasite-induced cysts localized by forming fibrous capsules in the liver. Fibrogenesis is the host immune response in the liver against these parasites. Hepatic stellate cells (HSCs) are localized perisinusoidal space also known as vitamin A-storing cells, characterize the important fibrogenic cell type. In this study, livers from 15 animals with hydatid cyst and 8 healthy animals were used. Hematoxylin and Eosin, masson trichrome staining were performed on the prepared liver sections. Microscopically, cysts were bordered eosinophilic necrotic debris blended with degenerate neutrophils, macrophages, eosinophils, lymphocytes, plasma cells and multinucleated giant cells, which extend into the adjacent fibrous connective tissue. In Masson trichrome staining, the fibrous connective tissue was observed surrounding of hydatid cyst. Glial fibrillary acidic protein (GFAP), collagen I, GFAP/collagen I, positive cells were investigated using either indirect single- or double-labeling immunohistochemical staining. The results indicated that anti-GFAP-positive staining was seen in areas including fibrous tissue just under the foreign body giant cells surrounding the cyst wall. In double immunohistochemical staining, it was observed that HSCs labeled with anti-GFAP antibody in the fibrous connective tissue also labeled anti-collagen I antibody. This study shows that HSCs may responsible for synthesis the collagen I in the development of parasitic fibrosis in cystic echinococcosis in the liver of cattle.

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          Author and article information

          Contributors
          ht_atmaca@yahoo.com
          Journal
          J Parasit Dis
          J Parasit Dis
          Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
          Springer India (New Delhi )
          0971-7196
          0975-0703
          6 June 2019
          December 2019
          : 43
          : 4
          : 576-582
          Affiliations
          [1 ] GRID grid.411506.7, ISNI 0000 0004 0596 2188, Department of Pathology, Faculty of Veterinary Medicine, , Balikesir University, ; Balikesir, Turkey
          [2 ] GRID grid.411047.7, ISNI 0000 0004 0595 9528, Department of Parasitology, Faculty of Veterinary Medicine, , Kırıkkale University, ; Kirikkale, Turkey
          [3 ] GRID grid.7256.6, ISNI 0000000109409118, Department of Internal Medicine, Faculty of Veterinary Medicine, , Ankara University, ; Ankara, Turkey
          [4 ] GRID grid.411047.7, ISNI 0000 0004 0595 9528, Department of Pathology, Faculty of Veterinary Medicine, , Kırıkkale University, ; Kirikkale, Turkey
          Article
          PMC6841830 PMC6841830 6841830 1129
          10.1007/s12639-019-01129-z
          6841830
          31749527
          © Indian Society for Parasitology 2019
          Categories
          Original Article
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          © Indian Society for Parasitology 2019

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