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      Flow cytometric measurement of intracellular cytokines.

      Journal of Immunological Methods
      Animals, Antigens, CD4, analysis, Antigens, Surface, Basophils, metabolism, Cytokines, Down-Regulation, Flow Cytometry, methods, Humans, Interferon-gamma, Interleukin-10, Interleukin-4, Kinetics, Leukocytes, Mononuclear, Mice, Quality Control, Th1 Cells, Th2 Cells

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          Abstract

          The identification of distinct T helper lymphocyte subsets (Th1/2) with polarised cytokine production has opened up new fields in immunobiology. Of the several alternative methods of monitoring cytokine production, flow cytometric analysis of intracellular staining has distinct advantages and pitfalls. It allows high throughput of samples and multiparameter characterisation of cytokine production on a single cell basis without the need for prolonged in vitro culture and cloning. However, these methods may cause important changes in cell surface phenotype which can make interpretation difficult.

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