The antibacterial effectiveness of 7 different endodontic sealers, AH Plus, Apexit
Plus, iRoot SP, Tubli Seal, Sealapex, Epiphany SE, and EndoRez against Enterococcus
faecalis was studied in vitro.
A modified direct contact test was used. Bacteria in suspension were exposed to the
materials for 2-60 minutes by using sealers that were freshly mixed or set for 1,
3, and 7 days. The pH values and contact angles of sterile water on sealers at different
times after setting were also measured.
Fresh iRoot SP killed all bacteria in 2 minutes, AH Plus in 5 minutes, EndoRez in
20 minutes, and Sealapex and Epiphany in 60 minutes. Freshly mixed Apexit Plus and
Tubli Seal failed to kill all bacteria at 60 minutes. For 1-day and 3-day samples,
iRoot SP and EndoRez had the strongest antibacterial activity, followed by Sealapex
and Epiphany; Tubli Seal and AH Plus did n'ot show any significant antibacterial activity.
Of all the samples, Apexit Plus had the lowest antimicrobial activity. The pH of the
sealers could not alone explain their antibacterial effect.
Fresh iRoot SP, AH Plus, and EndoRez killed E. faecalis effectively. IRoot SP and
EndoRez continued to be effective for 3 and 7 days after mixing. Sealapex and EndoRez
were the only ones with antimicrobial activity even at 7 days after mixing.