Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR.

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Abstract

This study developed an ultrasensitive method involving (1) gold nanoparticles encoded with a double-stranded DNA as the first signal amplification and goat anti-staphylococcal enterotoxin B (SEB) polyclonal antibody and (2) magnetic microparticles coated with anti-SEB monoclonal antibody to detect SEB. Both functionalized nanoparticles can capture SEB in a sandwich system. The released DNA barcodes were then characterized through SYBR Green real-time polymerase chain reaction and resulted in the second signal amplification. Under optimized experimental conditions, an ultrasensitive bio-barcode for SEB detection was established, and its detection limit could reach 0.269 pg mL-1, which was 1000-fold lower than that of conventional enzyme-linked/immunosorbent assay. The proposed method shows great potential in food security.

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Author and article information

Journal

Journal ID (iso-abbrev): Food Chem

Title: Food chemistry

Publisher: Elsevier BV

ISSN (Electronic): 1873-7072

ISSN (Print): 0308-8146

Publication date (Electronic): Jun 15 2019

Volume: 283

Affiliations

[1 ] College of Food Science and Engineering, Jilin University, Changchun 130022, PR China. Electronic address: xu_yanyang@yahoo.com.

[2 ] College of Food Science and Engineering, Jilin University, Changchun 130022, PR China.

[3 ] Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin 300050, PR China.

[4 ] School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300050, PR China.

[5 ] Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, PR China.

[6 ] Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin 300050, PR China. Electronic address: gaozhx@163.com.

Article

Publisher Item ID: S0308-8146(19)30076-7

DOI: 10.1016/j.foodchem.2018.12.128

PubMed ID: 30722881

SO-VID: d413263e-985f-49e1-bd24-8e6fb989f8c8

History

Keywords: Real-time PCR,Bio-barcode assay,Staphylococcal enterotoxin B

Data availability:

Keywords: Real-time PCR, Bio-barcode assay, Staphylococcal enterotoxin B

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