For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
19
views
18
references
 Top references
cited by
6
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      102
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Rapid and High-Throughput Detection of Highly Pathogenic Bacteria by Ibis PLEX-ID Technology

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potential bioterrorism-relevant microorganisms including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia mallei and pseudomallei, Brucella species, and Coxiella burnetii. DNA from 45 different reference materials with different formulations and different concentrations were chosen and sent to a service screening laboratory that uses the PCR/ESI-MS platform to provide a microbial identification service. The standard reference materials were produced out of a repository built up in the framework of the EU funded project “Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk” (EQADeBa). All samples were correctly identified at least to the genus level.

          Related collections

          Most cited references18

          • Record: found
          • Abstract: found
          • Article: not found

          Ibis T5000: a universal biosensor approach for microbiology

          David J. Ecker, Rangarajan Sampath, Christian Massire … (2008)
          The Ibis T5000 couples nucleic acid amplification to high-performance electrospray mass spectrometry and base-composition analysis and enables the identification and quantification of all known bacteria, all major groups of pathogenic fungi and the major families of viruses that cause disease in humans and animals. Here, Ecker and colleagues describe this new technology.
          Article 0 comments Cited 79 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance.

            Brian Libby, Stanley Crooke, Phillip Melton … (2005)
            Epidemic respiratory infections are responsible for extensive morbidity and mortality within both military and civilian populations. We describe a high-throughput method to simultaneously identify and genotype species of bacteria from complex mixtures in respiratory samples. The process uses electrospray ionization mass spectrometry and base composition analysis of PCR amplification products from highly conserved genomic regions to identify and determine the relative quantity of pathogenic bacteria present in the sample. High-resolution genotyping of specific species is achieved by using additional primers targeted to highly variable regions of specific bacterial genomes. This method was used to examine samples taken from military recruits during respiratory disease outbreaks and for follow up surveillance at several military training facilities. Analysis of respiratory samples revealed high concentrations of pathogenic respiratory species, including Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pyogenes. When S. pyogenes was identified in samples from the epidemic site, the identical genotype was found in almost all recruits. This analysis method will provide information fundamental to understanding the polymicrobial nature of explosive epidemics of respiratory disease.
            Article 0 comments Cited 50 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Direct broad-range detection of alphaviruses in mosquito extracts.

              Mark Eshoo, Chris A. Whitehouse, Scott T. Zoll … (2007)
              Members of the genus Alphavirus are a diverse group of principally mosquito-borne RNA viruses. There are at least 29 species and many more subtypes of alphaviruses and some are considered potential bioweapons. We have developed a multi-locus RT-PCR followed by electrospray ionization mass spectrometry (RT-PCR/ESI-MS) assay that uses the amplicon base compositions to detect and identify alphaviruses. A small set of primer pairs targeting conserved sites in the alphavirus RNA genome were used to amplify a panel of 36 virus isolates representing characterized Old World and New World alphaviruses. Base compositions from the resulting amplicons could be used to unambiguously determine the species or subtype of 35 of the 36 isolates. The assay detected, without culture, Venezuelan equine encephalitis virus (VEEV), Eastern equine encephalitis virus (EEEV), and mixtures of both in pools consisting of laboratory-infected and -uninfected mosquitoes. Further, the assay was used to detect alphaviruses in naturally occurring mosquito vectors collected from locations in South America and Asia. Mosquito pools collected near Iquitos, Peru, were found to contain an alphavirus with a very distinct signature. Subsequent sequence analysis confirmed that the virus was a member of the Mucambo virus species (subtype IIID in the VEEV complex). The assay we have developed provides a rapid, accurate, and high-throughput assay for surveillance of alphaviruses.
              Article 0 comments Cited 47 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                : Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (iso-abbrev): PLoS ONE
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, USA )
                ISSN (Electronic): 1932-6203
                Publication date Collection: 2012
                Publication date (Electronic): 29 June 2012
                Volume: 7
                Issue: 6
                Electronic Location Identifier: e39928
                Affiliations
                [1 ]Robert Koch-Institut, Berlin, Germany
                [2 ]Ibis Biosciences, Inc., an Abbott Company, Carlsbad, California, United States of America
                [3 ]Athogen, Inc., Irvine, California, United States of America
                Naval Research Laboratory, United States of America
                Author notes

                Conceived and designed the experiments: DJ US RG. Performed the experiments: DJ US CW KSL. Analyzed the data: DJ US CW KSL RH RS DJE RG. Contributed reagents/materials/analysis tools: RG DJE RS. Wrote the paper: RG RS DJE.

                Article
                Publisher ID: PONE-D-11-24686
                DOI: 10.1371/journal.pone.0039928
                PMC ID: 3386907
                PubMed ID: 22768173
                SO-VID: d41d55cb-2cc3-483c-89bc-abfa453fafc2
                Copyright © Jacob et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
                History
                Date received : 1 December 2011
                Date accepted : 4 June 2012
                Page count
                Pages: 6
                Categories
                Subject: Research Article
                Subject: Biology
                Subject: Microbiology
                Subject: Virology
                Subject: Emerging Viral Diseases
                Subject: Viral Disease Diagnosis
                Subject: Bacterial Pathogens
                Subject: Emerging Infectious Diseases
                Subject: Medicine
                Subject: Diagnostic Medicine
                Subject: Test Evaluation
                Subject: Infectious Diseases
                Subject: Bacterial Diseases
                Subject: Bacillus Cereus Infection
                Subject: Bacterial Pneumonia
                Subject: Burkholderia Infection
                Subject: Rickettsia Prowazekii
                Subject: Rickettsia Rickettsii
                Subject: Rickettsia
                Subject: Salmonella
                Subject: Shigellosis
                Subject: Tularemia
                Subject: Vibrio Vulnificus
                Subject: Yersinia Pestis
                Subject: Yersinia Pseudotuberculosis
                Subject: Yersiniosis

                ScienceOpen disciplines: Uncategorized
                Data availability:
                ScienceOpen disciplines: Uncategorized

                Comments

                Comment on this article

                scite_

                Similar content102

                See all similar

                Cited by6

                See all cited by

                Most referenced authors146

                See all reference authors