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      Modulation of Progesterone Secretion by Androgens in Porcine Granulosa Cell Cultures

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          Abstract

          The influence of testosterone, androstenedione and dihydrotestosterone (DHT) on the progesterone (P<sub>4</sub>) production by cultured porcine granulosa cells was studied in the presence or absence of gonadotropins. Porcine granulosa cells from large follicles (6-12 mm in diameter) were incubated for 2 days with 5% CO<sub>2</sub> in air with testosterone, androstenedione and DHT (10<sup>-12</sup>, 10<sup>-10</sup>, 10<sup>-8</sup> and 10<sup>-6</sup> M) in the presence or absence of luteinizing hormone (LH, 10 ng/ml) or follicle-stimulating hormone (FSH, 30 ng/ml). P<sub>4</sub> and pregnenolone (P<sub>5</sub>) in conditioned culture media were quantified by their specific RIAs. In the absence of gonadotropins, P<sub>4</sub> in media with androgens were not significantly different from controls. In the presence of LH, the addition of testosterone (10<sup>-10</sup>, 10<sup>-8</sup> M), androstenedione (10<sup>-8</sup> M) and DHT (10<sup>-8</sup> M) caused a significant 1.3- to 2.3-fold increase in P<sub>4</sub> over that caused by LH alone. In contrast, in the presence of FSH, testosterone (10<sup>-12</sup>,10<sup>-10</sup> M), androstenedione (10<sup>-12</sup>-10<sup>-6</sup> M) and DHT (10<sup>-6</sup> M) reduced the levels of P<sub>4</sub> by 22% to 41%. The addition of androgens with LH caused a significant increase in P<sub>5</sub>, while P<sub>5</sub> decreased in the presence of FSH. P<sub>4</sub>/P<sub>5</sub> ratios remained unchanged in the presence of both LH and FSH. These data suggest that the P<sub>4</sub> production by cultured porcine granulosa cells is modulated in a paracrine or an autocrine fashion by androgens in the presence of gonadotropins, and that androgens may exert their actions partly by altering the activity of cholesterol side chain cleavage enzymes.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          978-3-8055-5625-5
          978-3-318-01972-8
          1663-2818
          1663-2826
          1992
          1992
          03 December 2008
          : 37
          : Suppl 1
          : 25-31
          Affiliations
          Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan
          Article
          182342 Horm Res 1992;37:25–31
          10.1159/000182342
          1427624
          © 1992 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          New Aspects of the Physiology and Pathology of the Luteal Phase

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