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      Modulation of Progesterone Secretion by Androgens in Porcine Granulosa Cell Cultures

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          The influence of testosterone, androstenedione and dihydrotestosterone (DHT) on the progesterone (P<sub>4</sub>) production by cultured porcine granulosa cells was studied in the presence or absence of gonadotropins. Porcine granulosa cells from large follicles (6-12 mm in diameter) were incubated for 2 days with 5% CO<sub>2</sub> in air with testosterone, androstenedione and DHT (10<sup>-12</sup>, 10<sup>-10</sup>, 10<sup>-8</sup> and 10<sup>-6</sup> M) in the presence or absence of luteinizing hormone (LH, 10 ng/ml) or follicle-stimulating hormone (FSH, 30 ng/ml). P<sub>4</sub> and pregnenolone (P<sub>5</sub>) in conditioned culture media were quantified by their specific RIAs. In the absence of gonadotropins, P<sub>4</sub> in media with androgens were not significantly different from controls. In the presence of LH, the addition of testosterone (10<sup>-10</sup>, 10<sup>-8</sup> M), androstenedione (10<sup>-8</sup> M) and DHT (10<sup>-8</sup> M) caused a significant 1.3- to 2.3-fold increase in P<sub>4</sub> over that caused by LH alone. In contrast, in the presence of FSH, testosterone (10<sup>-12</sup>,10<sup>-10</sup> M), androstenedione (10<sup>-12</sup>-10<sup>-6</sup> M) and DHT (10<sup>-6</sup> M) reduced the levels of P<sub>4</sub> by 22% to 41%. The addition of androgens with LH caused a significant increase in P<sub>5</sub>, while P<sub>5</sub> decreased in the presence of FSH. P<sub>4</sub>/P<sub>5</sub> ratios remained unchanged in the presence of both LH and FSH. These data suggest that the P<sub>4</sub> production by cultured porcine granulosa cells is modulated in a paracrine or an autocrine fashion by androgens in the presence of gonadotropins, and that androgens may exert their actions partly by altering the activity of cholesterol side chain cleavage enzymes.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          03 December 2008
          : 37
          : Suppl 1
          : 25-31
          Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan
          182342 Horm Res 1992;37:25–31
          © 1992 S. Karger AG, Basel

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          Page count
          Pages: 7
          New Aspects of the Physiology and Pathology of the Luteal Phase


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