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      Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer

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          Abstract

          <p id="P1">The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis and response to therapy. We profiled transcriptomes of ~6,000 single cells from 18 head and neck squamous cell carcinoma (HNSCC) patients, including five matched pairs of primary tumors and lymph node metastases. Stromal and immune cells had consistent expression programs across patients. Conversely, malignant cells varied within and between tumors in their expression of signatures related to cell cycle, stress, hypoxia, epithelial differentiation, and partial epithelial-to-mesenchymal transition (p-EMT). Cells expressing the p-EMT program spatially localized to the leading edge of primary tumors. By integrating single-cell transcriptomes with bulk expression profiles for hundreds of tumors, we refined HNSCC subtypes by their malignant and stromal composition, and established p-EMT as an independent predictor of nodal metastasis, grade, and adverse pathologic features. Our results provide insight into the HNSCC ecosystem and define stromal interactions and a p-EMT program associated with metastasis. </p>

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          Author and article information

          Journal
          Cell
          Cell
          Elsevier BV
          00928674
          December 2017
          December 2017
          : 171
          : 7
          : 1611-1624.e24
          Article
          10.1016/j.cell.2017.10.044
          5878932
          29198524
          d469925c-92da-403e-a3d1-22d7603e5c8a
          © 2017

          http://www.elsevier.com/tdm/userlicense/1.0/

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