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      Expression of heat shock protein 47 is increased in remnant kidney and correlates with disease progression.

      International Journal of Experimental Pathology
      Animals, Blotting, Western, Collagen, genetics, metabolism, Disease Progression, Extracellular Matrix, pathology, Fluorescent Antibody Technique, Gene Expression, Glomerulosclerosis, Focal Segmental, etiology, HSP47 Heat-Shock Proteins, Heat-Shock Proteins, In Situ Hybridization, Kidney, Male, Nephrectomy, RNA, Messenger, Rats, Rats, Wistar

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          Abstract

          Glomerulosclerosis is characterized by accumulation of the mesangial extracellular matrix, including type I and V collagen. The processing for the collagens in the glomeruli may play a critical role for development of glomerulosclerosis. We examined the expression of heat shock protein 47 (HSP47), a collagen-binding molecular chaperone in the progressive glomerulosclerosis model. Subtotally nephrectomized rats, unlike sham-operated rats, developed focal and segmental glomerulosclerosis. Immunological staining demonstrated an increased expression of HSP47 which paralleled the expression of type I and IV collagen in the glomeruli of the nephrectomized rats as the glomerulosclerosis developed. The mRNA levels encoding type I and type IV collagen and HSP47 were increased 3.4 fold, 3.6 fold and 2.8 fold, respectively, at week 7 after nephrectomy. By in situ hybridization, the expression of HSP47 mRNA was determined to be localized to the glomeruli with segmental sclerosis. These results suggest that HSP47 may play a central role in the process of extracellular matrix accumulation during the development of glomerulosclerosis.

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