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Measurement of Cutaneous Inflammation: Estimation of Neutrophil Content with an Enzyme Marker
March 1982
March 1982
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Abstract
<p class="first" id="d9141644e61">We examined the hypothesis that myeloperoxidase
(MPO), a plentiful constituent of
neutrophils, might serve as a marker for tissue neutrophil content. To completely
extract MPO from either neutrophils or skin, hexadecyltrimethylammonium bromide (HTAB)
was used to solubilize the enzyme. With this detergent treatment, 97.8 +/- 0.2% of
total recoverable MPO was extracted from neutrophils with a single HTAB treatment;
93.1 +/- 1.0% was solubilized with a single treatment of skin. Neutrophil MPO was
directly related to neutrophil number; with the dianisidine-H2O2 assay as few as 10(4)
neutrophils could be detected. The background level of MPO within uninflamed tissue
was 0.385 +/- 0.018 units per gram of tissue, equivalent to only 7.64 +/- 0.36 X 10(5)
neutrophils. In experimental staphylococcal infection, skin specimens contained 34.8
+/- 3.8 units MPO per gram, equivalent to 8.55 +/- 0.93 X 10(7) neutrophils. These
studies demonstrate that MPO can be used as a marker for skin neutrophil content:
it is recoverable from skin in soluble form, and is directly related to neutrophil
number. Further, normal skin possesses a low background of MPO compared to that of
inflamed skin.
</p>