Marta Pokrywczynska 1 , * , Arkadiusz Jundzill 1 , Jan Adamowicz 1 , Tomasz Kowalczyk 2 , Karolina Warda 1 , Marta Rasmus 1 , Lukasz Buchholz 1 , Sandra Krzyzanowska 1 , Pawel Nakielski 3 , Tomasz Chmielewski 3 , Magdalena Bodnar 4 , Andrzej Marszalek 4 , 5 , Robert Debski 6 , Malgorzata Frontczak-Baniewicz 7 , Grzegorz Mikułowski 8 , Maciej Nowacki 1 , Tomasz A. Kowalewski 3 , Tomasz Drewa 1 , 9
27 August 2014
The purpose of this study was to compare: a new five-layered poly (L–lactide– co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide– co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10 6 cells/cm 2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10 6ADSCs; (II) SIS+ 3×10 6ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide– co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.