A very simple, fast, universally applicable and reproducible method to extract high
quality megabase genomic DNA from different organisms is described. We applied the
same method to extract high quality complex genomic DNA from different tissues (wheat,
barley, potato, beans, pear and almond leaves as well as fungi, insects and shrimps'
fresh tissue) without any modification. The method does not require expensive and
environmentally hazardous reagents and equipment. It can be performed even in low
technology laboratories. The amount of tissue required by this method is approximately
50-100 mg. The quantity and the quality of the DNA extracted by this method is high
enough to perform hundreds of PCR-based reactions and also to be used in other DNA
manipulation techniques such as restriction digestion, Southern blot and cloning.