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      Prevalence of Unclassified Bacteria in Tropical Coastal Waters of Malaysia Revealed by Metagenomic Approach

      brief-report
      Author(s): ,
      Publication date (Electronic):
      Journal: Genome Announcements
      Publisher: American Society for Microbiology

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          Abstract

          The metagenomes of marine prokaryotes from coastal seawaters in Malaysia are reported in this study. The investigation of the microbial communities using 16S rRNA gene amplicon metagenomic sequencing revealed that majority of the bacteria in the seawater samples remain unclassified.

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          Rapid isolation of high molecular weight plant DNA.

          M Murray, W. Thompson (1980)
          A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.
          Article 0 comments Cited 918 times – based on 0 reviews     Review now
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            Interactive effects of global climate change and pollution on marine microbes: the way ahead

            Francisco Coelho, Ana Santos, Joana Coimbra … (2013)
            Global climate change has the potential to seriously and adversely affect marine ecosystem functioning. Numerous experimental and modeling studies have demonstrated how predicted ocean acidification and increased ultraviolet radiation (UVR) can affect marine microbes. However, researchers have largely ignored interactions between ocean acidification, increased UVR and anthropogenic pollutants in marine environments. Such interactions can alter chemical speciation and the bioavailability of several organic and inorganic pollutants with potentially deleterious effects, such as modifying microbial-mediated detoxification processes. Microbes mediate major biogeochemical cycles, providing fundamental ecosystems services such as environmental detoxification and recovery. It is, therefore, important that we understand how predicted changes to oceanic pH, UVR, and temperature will affect microbial pollutant detoxification processes in marine ecosystems. The intrinsic characteristics of microbes, such as their short generation time, small size, and functional role in biogeochemical cycles combined with recent advances in molecular techniques (e.g., metagenomics and metatranscriptomics) make microbes excellent models to evaluate the consequences of various climate change scenarios on detoxification processes in marine ecosystems. In this review, we highlight the importance of microbial microcosm experiments, coupled with high-resolution molecular biology techniques, to provide a critical experimental framework to start understanding how climate change, anthropogenic pollution, and microbiological interactions may affect marine ecosystems in the future.
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              Author and article information

              Journal
              Journal ID (nlm-ta): Genome Announc
              Journal ID (iso-abbrev): Genome Announc
              Journal ID (hwp): ga
              Journal ID (pmc): ga
              Journal ID (publisher-id): GA
              Title: Genome Announcements
              Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
              ISSN (Electronic): 2169-8287
              Publication date (Electronic): 8 May 2014
              Publication date Collection: May-Jun 2014
              Volume: 2
              Issue: 3
              Electronic Location Identifier: e00419-14
              Affiliations
              [1]Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
              Author notes
              Address correspondence to Kok-Gan Chan, kokgan@ 123456um.edu.my .
              Article
              Publisher ID: genomeA00419-14
              DOI: 10.1128/genomeA.00419-14
              PMC ID: 4014694
              PubMed ID: 24812226
              SO-VID: d5fe5c76-bd34-4354-98a7-b536ed61965e
              Copyright © Copyright © 2014 Chan and Chong.
              License:

              This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

              History
              Date received : 14 April 2014
              Date accepted : 15 April 2014
              Page count
              Pages: 2
              Categories
              Subject: Prokaryotes
              Custom metadata
              cover-date May/June 2014
              access-type free

              ScienceOpen disciplines: Genetics
              Data availability:
              ScienceOpen disciplines: Genetics

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