Hemoglobin–oxygen affinity in high-altitude vertebrates: is there evidence for an adaptive trend?

ABSTRACT

In air-breathing vertebrates at high altitude, fine-tuned adjustments in hemoglobin (Hb)–O ₂ affinity provide an energetically efficient means of mitigating the effects of arterial hypoxemia. However, it is not always clear whether an increased or decreased Hb–O ₂ affinity should be expected to improve tissue O ₂ delivery under different degrees of hypoxia, due to the inherent trade-off between arterial O ₂ loading and peripheral O ₂ unloading. Theoretical results indicate that the optimal Hb–O ₂ affinity varies as a non-linear function of environmental O ₂ availability, and the threshold elevation at which an increased Hb–O ₂ affinity becomes advantageous depends on the magnitude of diffusion limitation (the extent to which O ₂ equilibration at the blood–gas interface is limited by the kinetics of O ₂ exchange). This body of theory provides a framework for interpreting the possible adaptive significance of evolved changes in Hb–O ₂ affinity in vertebrates that have colonized high-altitude environments. To evaluate the evidence for an empirical generalization and to test theoretical predictions, I synthesized comparative data in a phylogenetic framework to assess the strength of the relationship between Hb–O ₂ affinity and native elevation in mammals and birds. Evidence for a general trend in mammals is equivocal, but there is a remarkably strong positive relationship between Hb–O ₂ affinity and native elevation in birds. Evolved changes in Hb function in high-altitude birds provide one of the most compelling examples of convergent biochemical adaptation in vertebrates.

Abstract

Summary: Evolved changes in hemoglobin–oxygen affinity in high-altitude birds and mammals provide striking examples of convergent biochemical adaptation.