+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Identification of lactate as a driving force for prostanoid transport by prostaglandin transporter PGT.

      American Journal of Physiology - Renal Physiology

      genetics, metabolism, Biological Transport, drug effects, physiology, DNA-Binding Proteins, Deoxyglucose, pharmacology, Dinoprostone, pharmacokinetics, Dose-Response Relationship, Drug, Gene Expression, Glucose, Transfection, Glutamine, Glycolysis, HeLa Cells, Humans, Lactic Acid, Organic Anion Transporters, Oxidative Phosphorylation, Prostaglandins, Antiporters

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          We previously characterized the prostaglandin (PG) transporter PGT as an exchanger in which [(3)H]PGE(2) influx is coupled to the efflux of a countersubstrate. Here, we cultured HeLa cells that stably expressed human PGT under conditions known to favor glycolysis (glucose as a carbon source) or oxidative phosphorylation (glutamine as a carbon source) and studied the effect on PGT-mediated [(3)H]PGE(2) influx. PGT-expressing cells grown in glutamine exhibited a 2- to 4-fold increase in [(3)H]PGE(2) influx compared with the antisense control, whereas cells grown in glucose exhibited a 14-fold increase. In the presence of 10 vs. 25 mM glucose during the uptake, there was a dose-dependent increment in [(3)H]PGE(2) influx. Cis inhibition of [(3)H]PGE(2) influx occurred with lactate at physiological concentrations (apparent K(m) = 48 +/- 12 mM). Preloading with lactate caused a dose-dependent trans stimulation of PGT-mediated [(3)H]PGE(2) uptake, and external lactate caused trans stimulation of PGT-mediated [(3)H]PGE(2) release. Together, these data are consistent with PGT-mediated PG-lactate exchange. Cells engaged in glycolysis would then be poised energetically for prostanoid uptake by means of PGT.

          Related collections

          Author and article information



          Comment on this article