A new rhodamine B hydrazone derivative (probe L) was synthesized and characterized. The probe L had sufficiently satisfactory selective response to Fe(3+) and Cu(2+) ions among various interferential metal ions, and high sensitivity with the detection limit of 4.63×10(-9)M and 5.264×10(-7)M for Fe(3+) and Cu(2+) ions, respectively. In the presence of Fe(3+), the probe L exhibited turn-on orange fluorescence accompanied by color change from colorless to pink. Toward Cu(2+), the probe L showed significant color change from colorless to red purple. These remarkable orange fluorescence and color change made probe L suitable naked-eye identify for Fe(3+) and Cu(2+) ions. By means of Job's plot, Benesi-Hildebrand studies and FTIR spectra, both 1:1 binding modes (L-Fe(3+) and L-Cu(2+)) were confirmed. The coordination mechanism and turn on/off fluorescence for L-Fe(3+) and L-Cu(2+) complexes were well explained by theoretical calculations. Moreover, probe L could be used as a quick, simple, visual test strip for Fe(3+) and Cu(2+) detection.