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      Pfaffia paniculata extract improves red blood cell deformability in sickle cell patients.

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          Abstract

          The aim of the present study was to test the effects of Pfaffia paniculata (PP) extract on the red blood cell (RBC) rheological properties of patients with sickle cell disease (SCD) and healthy (AA) individuals. Blood from 7 SCD and 4 AA individuals were collected in EDTA tubes. Washed RBCs were incubated with various concentration of PP extract: 0.0, 0.2 or 0.5 mg/ml of PP solution for 5 hrs at 37°C. RBC deformability was measured by ektacytometry at 9 shear stresses ranging from 0.3 to 30 Pa, and RBC aggregation properties were determined by laser-backscattered techniques. Because RBCs from SCD patients are fragile, a stability test was also performed to test for the fragility of RBC exposed to a constant shear stress (70 Pa) for 10 min. While RBC deformability was not improved by the use of PP extract in AA, we noted an improvement of this parameter in patients with SCD between the 0.0 and 0.5 mg/ml conditions. In contrast to AA RBCs, the fragility of SCD RBCs was not affected by PP extract. In conclusion, this study demonstrates the beneficial effects, in-vitro, of PP extract on the RBC deformability of SCD patients, notably at high shear stress (a shear stress condition usually found in capillaries).

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          Author and article information

          Journal
          Clin Hemorheol Microcirc
          Clinical hemorheology and microcirculation
          IOS Press
          1875-8622
          1386-0291
          Sep 25 2015
          : 62
          : 4
          Affiliations
          [1 ] Inserm U 1134, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.
          [2 ] Laboratory of Excellence GR-Ex, Paris, France.
          [3 ] Laboratoire ACTES-EA3596, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe.
          [4 ] Department of Medicine, University of Pecs Medical School, Pecs, Hungary.
          [5 ] Unité Transversale de la Drépanocytose, CHU de Pointe-à-Pitre, Pointe-à-Pitre, Guadeloupe.
          [6 ] Service d'Immunologie-Hématologie, CHU de Pointe à Pitre, Pointe-à-Pitre, Guadeloupe.
          [7 ] Research Laboratories, GDP Ayurvedic University (GDPAU), New Brunswick, NJ, USA.
          [8 ] Institut Universitaire de France, Paris, France.
          [9 ] Laboratoire CRIS EA647, Equipe "Biologie Vasculaire et Globule Rouge", Université de Lyon (UCBL1), France.
          [10 ] Cardeza Foundation, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
          Article
          CH1972
          10.3233/CH-151972
          26444603
          d756452e-41ad-411c-bd2a-80de0e6a8c9a
          History

          Pfaffia paniculata,blood rheology,sickle cell anemia,sickle cell disease

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