A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera‐ than Coleoptera‐targeted Bacillus thuringiensis strains

Abstract

BACKGROUND

The pine weevil ( Hylobius abietis) is a major forest regeneration pest causing high levels of seedling mortality and economic losses. Current management relies on silviculture, stem coatings and insecticides. Here we evaluated for the first time the effects of Bacillus thuringiensis ( Bt) strains on H. abietis adults: two producing the Coleoptera‐targeted toxins Cry3Aa ( Bt tenebrionis NB‐176) and Cry8Da ( Bt galleriae SDS‐502), and one producing the Diptera‐targeted Cry10A ( Bt israelensis AM65‐52). Choice and nonchoice assays using individual and mixtures of Bt formulations, containing these strains respectively, were conducted.

RESULTS

We found that Bt had toxic and lethal effects on H. abietis, but effects varied with strain and formulation concentration. The Diptera‐targeted Bt israelensis had the most negative effects on weevil weight, feeding and mortality (70–82% feeding reduction, 65–82% greater mortality than control), whereas the effect was lower for the Coleoptera‐specific Bt tenebrionis (38–42%; 37–42%) and Bt galleriae (11–30%; 15–32%). Reduced weevil feeding was observed after 3 days, and the highest mortality occurred 7–14 days following Bt exposure. However, we found no synergistic toxic effects, and no formulation combination was better than Bt israelensis alone at reducing consumption and survival. Also, pine weevils were not deterred by Bt, feeding equally on Bt‐treated and non‐ Bt treated food.

CONCLUSION

There is potential to develop forest pest management measures against H. abietis that include Bt, but only the Diptera‐targeted Bt israelensis would provide effective seedling protection. Its Diptera‐specificity may need reconsideration, and evaluation of other Bt strains/toxins against H. abietis would be of interest. © 2020 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Abstract

Effects of Bacillus thuringiensis on the pine weevil Hylobius abietis were evaluated for the first time. A Diptera‐targeted strain was most toxic and could enhance forest protection against H. abietis.