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      Is there any cardioprotective role of Taurine during cold ischemic period following global myocardial ischemia?

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          Abstract

          Background

          The aim of the present study was to investigate the cardioprotective effect of Taurine on the donor hearts during cold ischemic period.

          Methods

          32 rats were divided into four groups (sham, taurine, ischemia, treatment group, 8 rats in each). All rats were fed with rat food for three weeks. Taurine and treatment groups were given a 200 mg/kg/day dose of Taurine by oral gavage besides rat feed. Cardiectomy was performed in all rats after three weeks. In ischemia and treatment groups, harvested hearts were kept in 0.9% sodium chloride at +4 degrees C for 5 hours. Tissue samples were taken from left ventricle in all groups. These samples were evaluated by histopathologic and biochemical examination.

          Results

          In the present study results of the biochemical and histopathological examination reveals the protective effects of Taurine. As a marker of lipid peroxidation, Malondialdehyde (MDA) levels in ischemia group were significantly higher than both Sham and Taurine groups. MDA values were recorded; 3.62 ± 0.197 in the sham group, 2.07 ± 0.751 in the Taurine group, 9.71 ± 1.439 in the ischemia group and 7.68 ± 1.365 in the treatment group. MDA levels decreased in treatment group. (p < 0.05) In accordance with MDA findings, while superoxide dismutase and glutathione peroxidase levels decreased in ischemia group, they increased in treatment group. (p < 0.05) There was no differences in Catalase (CAT) enzyme level between treatment and ischemia group (p = 1.000). CAT level results were recorded; 7.08 ± 0.609 in the sham group, 6.15 ± 0.119 in the Taurine group, 5.02 ± 0.62 in the ischemia group, and 5.36 ± 0.384 in the treatment group. Less intracellular edema and inflammatory cell reaction were observed in histologic examination in favor of treatment group. (p < 0.01)

          Conclusion

          Taurine decreased myocardial damage during cold ischemic period following global myocardial ischemia.

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          Most cited references29

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          • Abstract: found
          • Article: not found

          A simple method for clinical assay of superoxide dismutase.

          This assay for superoxide dismutase (SOD, EC 1.15.1.1) activity involves inhibition of nitroblue tetrazolium reduction, with xanthine-xanthine oxidase used as a superoxide generator. By using a reaction terminator, we can determine 40 samples within 55 min. One unit of activity of pure bovine liver Cu,ZnSOD and chicken liver MnSOD was expressed by 30 ng and 500 ng of protein, respectively. The mean concentrations of Cu,ZnSOD as measured by this method in blood from normal adults were 242 (SEM 4) mg/L in erythrocytes, 548 (SEM 20) micrograms/L in serum, and 173 (SEM 11) micrograms/L in plasma. The Cu,ZnSOD concentrations in serum and plasma of patients with cancer of the large intestine tended to be less and greater than these values, respectively, but not statistically significantly so.
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            Oxygen-derived free radicals in postischemic tissue injury.

            J M McCord (1985)
            It is now clear that oxygen-derived free radicals play an important part in several models of experimentally induced reperfusion injury. Although there are certainly multiple components to clinical ischemic and reperfusion injury, it appears likely that free-radical production may make a major contribution at certain stages in the progression of the injury. The primary source of superoxide in reperfused reoxygenated tissues appears to be the enzyme xanthine oxidase, released during ischemia by a calcium-triggered proteolytic attack on xanthine dehydrogenase. Reperfused tissues are protected in a variety of laboratory models by scavengers of superoxide radicals or hydroxyl radicals or by allopurinol or other inhibitors of xanthine oxidase. Dysfunction induced by free radicals may thus be a major component of ischemic diseases of the heart, bowel, liver, kidney, and brain.
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              • Article: not found

              Malondialdehyde determination as index of lipid peroxidation.

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                Author and article information

                Journal
                J Cardiothorac Surg
                Journal of Cardiothoracic Surgery
                BioMed Central
                1749-8090
                2011
                18 March 2011
                : 6
                : 31
                Affiliations
                [1 ]Gülhane Military Medical Academy, Department of Cardiovascular Surgery, 06010, Etlik, Ankara, Turkey
                [2 ]Gülhane Military Medical Academy, Department of Thoracic Surgery, 06010, Etlik, Ankara, Turkey
                [3 ]Gazi University, Faculty of Commerce and Tourism Education, Department of Computer Applications Training, 06830, Gölbaşı, Ankara, Turkey
                [4 ]Gülhane Military Medical Academy, Department of Pathology, 06010, Etlik, Ankara, Turkey
                [5 ]Gülhane Military Medical Academy, Department of Biochemistry, 06010, Etlik, Ankara, Turkey
                Article
                1749-8090-6-31
                10.1186/1749-8090-6-31
                3068941
                21418563
                d7cc976f-d881-4864-aa48-ae2dbf21b5d6
                Copyright ©2011 Sahin et al; licensee BioMed Central Ltd.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 7 December 2010
                : 18 March 2011
                Categories
                Research Article

                Surgery
                Surgery

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