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      A portable SERS method for the determination of uric acid using a paper-based substrate and multivariate curve resolution

      1 , 2 , 3 , 4 , 1 , 2 , 3 , 4
      The Analyst
      Royal Society of Chemistry (RSC)

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          Abstract

          This paper presents a portable quantitative method for the on-site determination of uric acid in urine using surface-enhanced Raman spectroscopy (SERS) and gold nanoparticle-coated paper as a substrate.

          Abstract

          This paper presents a portable quantitative method for the on-site determination of uric acid in urine using surface-enhanced Raman spectroscopy (SERS) and gold nanoparticle-coated paper as a substrate. A procedure was developed for the rapid preparation of cost-effective SERS substrates that enabled the adequate control of a homogeneous active area and the use of small quantities of gold nanoparticles per substrate. The standard addition method and multivariate curve resolution-alternating least squares (MCR-ALS) were applied to compensate for the matrix effect and to address overlapping bands between uric acid and interference SERS spectra. The proposed methodology demonstrated better performance than conventional univariate methods (in terms of linearity, accuracy and precision), a wide linear range (0–3.5 mmol L −1) and an adequate limit of detection (0.11 mmol L −1). For the first time, a portable SERS method coupled with chemometrics was developed for the routine analysis of uric acid at clinically relevant concentrations with minimal sample preparation and easy extension for the on-site determination of other biomarkers in complex sample matrices.

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          Surface enhanced Raman spectroscopy: analytical, biophysical and life science applications

          Etchegoin (2010)
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            Author and article information

            Journal
            ANALAO
            The Analyst
            Analyst
            Royal Society of Chemistry (RSC)
            0003-2654
            1364-5528
            2016
            2016
            : 141
            : 6
            : 1966-1972
            Affiliations
            [1 ]Institute of Chemistry
            [2 ]University of Campinas
            [3 ]13081-970 Campinas
            [4 ]Brazil
            Article
            10.1039/C5AN02398J
            d815e7f2-2554-415a-a30a-882d7b83a782
            © 2016
            Product
            Self URI (article page): http://xlink.rsc.org/?DOI=C5AN02398J

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