Apoptosis and Myofibroblast Expression in Human Glomerular Disease: A Possible Link with Transforming Growth Factor-Beta-1

Abstract

Background/Aims: The pathophysiological pathways involved in the pathogenesis and evolution of renal fibrosis, have not been fully elucidated. Transforming growth factor-beta₁ (TGF-β₁) is involved in the development of renal scarring. Apoptosis is responsible for intrinsic cell deletion observed in end-stage kidney disease. Myofibroblasts are involved in the development of renal fibrosis. This study investigates whether there is a potential relationship between apoptosis, myofibroblast infiltration and TGF-β₁ expression in the kidney of patients with glomerulonephritis (GN). Methods: Forty patients with various types of GN were included in the study. Myofibroblasts and TGF-β₁ positive cells were detected in kidney biopsies by immunohistochemistry, while apoptotic cells were detected by the in situ end labelling of fragmented DNA. Results: Myofibroblasts were identified in the glomeruli of some patients with severe mesangioproliferative GN and glomerulosclerosis but a more intensive myofibroblast expression was found in the renal interstitium. TGF-β₁ was expressed in the cytoplasm of tubular epithelial cells, in the renal interstitium and in the glomeruli of patients with GN. Apoptotic cells were mainly detected in the tubules and interstitium and were present in areas with intense myofibroblast infiltration. Positive correlations were observed between the intensity of myofibroblast expression in the interstitium and apoptosis in the tubulointerstitial area (r = 0.521, p < 0.01) as well as TGF-β₁ expression (r = 0.462, p < 0.05) and degree of renal impairment (r = 0.430, p < 0.05). Conclusions: These observations suggest that myofibroblast infiltration and apoptosis along with TGF-β₁ expression are associated with the development of interstitial fibrosis in patients with glomerular disease.

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 2002

Publication date (Print): October 2002

Publication date (Electronic): 02 September 2002

Volume: 92

Issue: 2

Pages: 287-296

Affiliations

Departments of ^aInternal Medicine-Nephrology and ^bPathology, University of Patras, Patras, Greece; ^cSheffield Kidney Institute, NGH, Sheffield, UK; ^dDepartment of Pathology, General Hospital ‘Evangelismos’, Athens, Greece

Article

Publisher ID: 63293 Other ID: Nephron 2002;92:287–296

DOI: 10.1159/000063293

PubMed ID: 12218305

Copyright statement: © 2002 S. Karger AG, Basel

License:

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Page count

Figures: 4, Tables: 2, References: 31, Pages: 10

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Categories

Subject: Original Paper